ABSTRACT In the first stage of the manuscript, we aimed to examine antioxidant capacity and anticholinergic properties of avocado (Folium perseae) leaves. Avocado leaf was extracted by water (WEFP) and ethyl alcohol (EEFP) and antioxidant activity was determined using by several antioxidant assays including DPPH· and ABTS•+ radical scavenging assays, Cu2+-Cu+ reducing, Fe3+-Fe2+ reducing, and FRAP reducing activities. Avocado leaf extracts demonstrated antioxidant activity and anticholinergic activities, while α-tocopherol, BHT, trolox, and BHA were used as positive antioxidant controls. In the second part of this study, the inhibition effects of WEFP and EEFP were valuated against acetylcholinesterase and butyrylcholinesterase enzymes, which catalyze the breakdown of choline esters (i.e. neurotransmitters). This study obviously showed that avocado leaf extracts had effective antioxidant, antiradical, and anticholinergic influences.

中文翻译：

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鳄梨（Folium perseae）叶的抗胆碱能和抗氧化活性——LC-MS/MS 分析的植物化学成分

摘要在手稿的第一阶段，我们旨在检查鳄梨（Folium perseae）叶的抗氧化能力和抗胆碱能特性。鳄梨叶用水 (WEFP) 和乙醇 (EEFP) 提取，抗氧化活性通过多种抗氧化试验测定，包括 DPPH· 和 ABTS•+ 自由基清除试验、Cu2+-Cu+ 还原、Fe3+-Fe2+ 还原和 FRAP 还原活性. 鳄梨叶提取物显示出抗氧化活性和抗胆碱能活性，而 α-生育酚、BHT、trolox 和 BHA 被用作抗氧化阳性对照。在本研究的第二部分，WEFP 和 EEFP 对乙酰胆碱酯酶和丁酰胆碱酯酶的抑制作用进行了评估，这些酶催化胆碱酯（即神经递质）的分解。