Abstract Introduction Although peripheral blood analysis has become increasingly automated, microscopy is the only available method for the diagnosis of anisocytosis and poikilocytosis. The aims of the study were to compare RBC volume data obtained with two different analysers and by manual assessment of smears and to compare this data between dogs in various stages of heart failure secondary to degenerative mitral valvular (DMV) disease. The impact of diuretic administration on RBC morphology was also assessed. Material and Methods Sixty-eight dogs, 56 in different stages of DMV disease and 12 as healthy controls, were studied. Impedance and flow cytometry haematological analyses were performed for each animal. Additionally, two smears were prepared for manual analysis. RBC structure, staining, and size differences were recorded. Results There were no significant differences between the blood morphological parameters assessed using haematological analysers nor between dogs receiving diuretic treatment and those not treated. Based on the manual smear, significantly higher erythrocyte anisocytosis was observed in the dogs with symptomatic DMV disease than in the control group. Conclusion Haematological analysers based on impedance and flow cytometry provide reliable and comparable morphological results in dogs with heart failure. However, microscopic assessment of blood smears is a more reliable tool to detect erythrocyte anisocytosis.

中文翻译：

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退行性二尖瓣病不同阶段犬红细胞参数分析

摘要介绍虽然外周血分析变得越来越自动化，但显微镜检查是诊断红细胞异常和异红细胞增多症的唯一可用方法。该研究的目的是比较使用两种不同的分析仪和通过涂片的手动评估获得的红细胞体积数据，并在继发于退行性二尖瓣 (DMV) 疾病的心力衰竭不同阶段的狗之间比较这些数据。还评估了利尿剂给药对红细胞形态的影响。材料和方法 研究了 68 只狗，其中 56 只处于 DMV 疾病的不同阶段，12 只作为健康对照。对每只动物进行阻抗和流式细胞术血液学分析。此外，还准备了两个涂片用于手动分析。记录红细胞结构、染色和大小差异。结果 使用血液分析仪评估的血液形态参数之间没有显着差异，接受利尿剂治疗的狗与未治疗的狗之间也没有显着差异。根据手工涂片，在有症状的 DMV 疾病的狗中观察到的红细胞不均匀性显着高于对照组。结论基于阻抗和流式细胞术的血液分析仪在心力衰竭犬中提供了可靠且可比较的形态学结果。然而，血涂片的显微镜评估是检测红细胞异红细胞增多症的更可靠工具。与对照组相比，在患有症状性 DMV 疾病的狗中观察到显着更高的红细胞异红细胞增多症。结论基于阻抗和流式细胞术的血液分析仪在心力衰竭犬中提供了可靠且可比较的形态学结果。然而，血涂片的显微镜评估是检测红细胞异红细胞增多症的更可靠工具。与对照组相比，在患有症状性 DMV 疾病的狗中观察到显着更高的红细胞异红细胞增多症。结论基于阻抗和流式细胞术的血液分析仪在心力衰竭犬中提供了可靠且可比较的形态学结果。然而，血涂片的显微镜评估是检测红细胞异红细胞增多症的更可靠工具。