Abstract We aimed to investigate the impact of oxytocin on serum thiol/disulphide and malonylyldialdehyde (MDA)/glutathione balance under acute stress (AS) and chronic stress (CS) exposure in rats. Animals were allocated into control (C), AS and CS groups, then the groups subdivided as intranasal oxytocin or saline applied groups, randomly. Animals in the AS or CS groups were exposed to combined cold-immobilisation stress. Salivary corticosterone levels and elevated plus maze (EPM) scores were used to assess stress response. MDA, glutathione, thiol-disulphide levels were measured in the serum samples. Oxytocin treatment attenuated stress response regardless of the stress duration verified by lower corticosterone level and favorable profile in EPM parameters measured. Furthermore, oxytocin modulated oxidant profile suggesting lowered oxidant stress with decreased serum MDA/glutathione and disulfide/native thiol ratios. Oxytocin improves the response of organism to stress via both its anxiolytic and antioxidant effects. That’s why it can be considered as a protective measure to employ methods to increase endogenous oxytocin and/or to apply exogenous oxytocin to prevent stress-induced increase in oxidant stress, which plays a pivotal role in the pathogenesis of various stress-related diseases.

中文翻译：

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催产素对应激大鼠硫醇/二硫化物和丙二醛/谷胱甘肽稳态的影响

摘要 我们旨在研究催产素对大鼠急性应激（AS）和慢性应激（CS）暴露下血清硫醇/二硫化物和丙二醛（MDA）/谷胱甘肽平衡的影响。将动物分为对照组（C）、AS 和 CS 组，然后将这些组随机细分为鼻内催产素或生理盐水应用组。AS 或 CS 组的动物暴露于联合冷固定应激。唾液皮质酮水平和升高的十字迷宫 (EPM) 分数用于评估压力反应。测量血清样品中的 MDA、谷胱甘肽、硫醇二硫化物水平。无论通过较低的皮质酮水平和 EPM 参数中的有利特征证实的应激持续时间如何，催产素治疗都会减弱应激反应。此外，催产素调节氧化剂谱表明随着血清 MDA/谷胱甘肽和二硫化物/天然硫醇比率的降低，氧化应激降低。催产素通过其抗焦虑和抗氧化作用改善生物体对压力的反应。这就是为什么可以考虑采用增加内源性催产素和/或应用外源性催产素的方法来防止应激引起的氧化应激增加的保护措施，氧化应激在各种应激相关疾病的发病机制中起着举足轻重的作用。