Given that the extent to which genetics alters the metabolomic profile of tissues is still poorly understood, the current study aimed to characterize and investigate the metabolite profiles of brain, liver, kidney and skeletal muscle of two common mouse inbred strains (BALB/c, C57BL/6) and one outbred stock (CD1) for strain-specific differences. Male mice (n = 15) at the age of 12 weeks were used: BALB/c (n = 5), C57BL/6 (n = 5) and CD1 (n = 5). Solid phase microextraction (SPME) was applied for the extraction of analytes from the tissues. SPME fibers (approximately 0.2 mm in diameter) coated with a biocompatible sorbent (4 mm length of hydrophilic-lipophilic balanced particles) were inserted into each organ immediately after euthanasia. Samples were analyzed using liquid chromatography coupled to a Q-Exactive Focus Orbitrap mass spectrometer. Distinct interstrain differences in the metabolomic patterns of brain and liver tissue were revealed. The metabolome of kidney and muscle tissue in BALB/c mice differed greatly from C57BL/6 and CD1 strains. The main compounds differentiating all the targeted organs were alpha-amino acids, purine nucleotides and fatty acid esters. The results of the study indicate that the baseline metabolome of organs, as well as different metabolic pathways, vary widely among general-purpose models of laboratory mice commonly used in biomedical research.

中文翻译：

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基于SPME-LC-HRMS的不同菌株小鼠器官代谢组学特征比较。

鉴于对遗传学改变组织代谢组学特征的程度仍知之甚少，本研究旨在表征和研究两种常见小鼠近交系（BALB / c，C57BL）的脑，肝，肾和骨骼肌的代谢特征/ 6）和一只近交种（CD1），以区分菌株。使用12周龄的雄性小鼠（n = 15）：BALB / c（n = 5），C57BL / 6（n = 5）和CD1（n= 5）。固相微萃取（SPME）用于从组织中提取分析物。安乐死后立即将涂有生物相容性吸附剂（长度为4 mm的亲水-亲脂平衡颗粒）的SPME纤维（直径约0.2 mm）插入每个器官。使用与Q-Exactive Focus Orbitrap质谱仪耦合的液相色谱仪分析样品。揭示了大脑和肝脏组织的代谢组学模式中明显的株间差异。BALB / c小鼠的肾脏和肌肉组织的代谢组与C57BL / 6和CD1株差异很大。区分所有目标器官的主要化合物是α-氨基酸，嘌呤核苷酸和脂肪酸酯。研究结果表明，器官的基础代谢组以及不同的代谢途径，