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Molecular identification of forensically important beetles in Saudi Arabia based on mitochondrial 16 s rRNA gene
Entomological Research ( IF 1.2 ) Pub Date : 2020-06-16 , DOI: 10.1111/1748-5967.12438
Reem Alajmi 1 , Rewaida Abdel‐Gaber 1, 2 , Rania Haddadi 1
Affiliation  

Forensic science uses scientific methods to help the scientists who study evidence to assist in the solving of crimes. Coleoptera is the most diverse and speciose group of insects that have an important role in many scientific fields especially forensic entomology. In addition, it is difficult to morphologically identify and discriminate between them. In the present study, the molecular analysis using mitochondrial DNA information was conducted to swiftly and accurately identify the recovered Coleoptera species. A molecular identification method involving a 221‐bp segment of the 16 s ribosomal RNA (16 s rRNA) gene from three beetle species, collected from a rabbit carcass, was evaluated. The analysis with maximum likelihood method recovered a generally well supported phylogeny, with most currently accepted taxa and species groups as monophyletic. These results will be instrumental for the implementation of the Saudian database of forensically relevant beetles.

中文翻译:

基于线粒体16s rRNA基因的沙特阿拉伯法医重要甲虫分子鉴定

法医学用科学的方法帮助研究证据的科学家协助破案。鞘翅目是最多样化、最特殊的昆虫类群,在许多科学领域,尤其是法医昆虫学中发挥着重要作用。此外,很难在形态上识别和区分它们。在本研究中,使用线粒体 DNA 信息进行分子分析,以快速准确地识别回收的鞘翅目物种。评估了一种涉及 16 s 核糖体 RNA (16 s rRNA) 基因的 221 bp 片段的分子鉴定方法,该基因来自三种甲虫物种,从兔子尸体中收集。使用最大似然法进行的分析恢复了普遍得到充分支持的系统发育,大多数目前接受的分类群和物种组为单系。
更新日期:2020-06-16
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