A Magnetic Iron Oxide/Polydopamine Coating Can Improve Osteogenesis of 3D-Printed Porous Titanium Scaffolds with a Static Magnetic Field by Upregulating the TGFβ-Smads Pathway.,Advanced Healthcare Materials

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A Magnetic Iron Oxide/Polydopamine Coating Can Improve Osteogenesis of 3D-Printed Porous Titanium Scaffolds with a Static Magnetic Field by Upregulating the TGFβ-Smads Pathway.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-06-16 , DOI: 10.1002/adhm.202000318
Zhenfei Huang _{1,

2} , Yu He _{1,

3} , Xiao Chang ₁ , Jieying Liu ₄ , Lingjia Yu _{1,

5} , Yuanhao Wu ₄ , Yaqian Li ₄ , Jingjing Tian ₄ , Lin Kang ₄ , Di Wu ₁ , Hai Wang ₁ , Zhihong Wu _{4,

6} , Guixing Qiu ₁

Affiliation

3D‐printed porous titanium–aluminum–vanadium (Ti6Al4V, pTi) scaffolds offer surgeons a good option for the reconstruction of large bone defects, especially at the load‐bearing sites. However, poor osteogenesis limits its application in clinic. In this study, a new magnetic coating is successfully fabricated by codepositing of Fe₃O₄ nanoparticles and polydopamine (PDA) on the surface of 3D‐printed pTi scaffolds, which enhances cell attachment, proliferation, and osteogenic differentiation of hBMSCs in vitro and new bone formation of rabbit femoral bone defects in vivo with/without a static magnetic field (SMF). Furthermore, through proteomic analysis, the enhanced osteogenic effect of the magnetic Fe₃O₄/PDA coating with the SMF is found to be related to upregulate the TGFβ‐Smads signaling pathway. Therefore, this work provides a simple protocol to improve the osteogenesis of 3D‐printed porous pTi scaffolds, which will help their application in clinic.

中文翻译：

磁性氧化铁/聚多巴胺涂层可以通过上调TGFβ-Smads途径来改善3D打印的具有静磁场的多孔钛支架的成骨性。

3D打印的多孔钛-铝-钒（Ti6Al4V，pTi）支架为外科医生提供了重建大型骨缺损（尤其是在承重部位）的好选择。然而，不良的成骨作用限制了其在临床中的应用。在这项研究中，通过在3D打印的pTi支架表面上共沉积Fe ₃ O ₄纳米颗粒和聚多巴胺（PDA），成功制造了一种新的磁性涂层，从而增强了hBMSCs的细胞附着，增殖和成骨分化。在有/没有静磁场（SMF）的情况下，兔股骨缺损的骨骼形成。此外，通过蛋白质组学分析，磁性Fe ₃ O ₄的增强成骨作用/ PDA与SMF涂层被发现上调TGF是相关的β -Smads信号传导途径。因此，这项工作为改善3D打印的多孔pTi支架的成骨性提供了一个简单的协议，这将有助于它们在临床中的应用。

更新日期：2020-07-22

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