All-Aqueous-Processed Injectable In Situ Forming Macroporous Silk Gel Scaffolds for Minimally Invasive Intracranial and Osteological Therapies.,Advanced Healthcare Materials

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All-Aqueous-Processed Injectable In Situ Forming Macroporous Silk Gel Scaffolds for Minimally Invasive Intracranial and Osteological Therapies.
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2020-06-16 , DOI: 10.1002/adhm.202000879
Keyin Liu ₁ , Zhen Fan ₂ , Tianji Wang ₃ , Zhiheng Gao ₁ , Junjie Zhong ₂ , Geng Xiang ₃ , Wei Lei ₃ , Zhifeng Shi ₂ , Yafei Feng ₃ , Ying Mao ₂ , Tiger H Tao _{1,

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Affiliation

Hydrogels are widely utilized in regenerative medicine for drug delivery and tissue repair due to their superior biocompatibility and high similarity to the extracellular matrix. For minimally invasive therapies, in situ forming gel scaffolds are desirable, but technical challenges remain to be overcome to achieve the balance between tissue‐like strength and cell‐sized porosity, especially for intracranial and osteological therapies. Here, a new method—inspired by the liquid crystalline spinning process in natural silk fibers—is reported for preparing injectable silk gel scaffolds with favorable preclinical efficacy and unique characteristics including 1) in situ gelling for minimally invasive surgeries, 2) controllable porosity for efficient cellular infiltration and desirable degradation, 3) resilient and tunable mechanical properties that are compatible with the modulus regime of native soft tissues, and 4) all‐aqueous processing that avoids toxic solvents and enables facile loading of bioactive agents. Moreover, hierarchically structured heterogeneous silk gel scaffolds with variable porosity and bioactive agent gradients within 3D matrices can be achieved for sustained drug release and guided tissue regeneration. Preclinical efficacy studies in rodent models show efficient bacterium and glioma inhibition and positive effects on bone regeneration and vascularization.

中文翻译：

全水处理可注射的原位形成大孔丝绸凝胶支架，用于微创颅内和骨科治疗。

水凝胶由于其优越的生物相容性和与细胞外基质的高度相似性而被广泛用于再生医学中的药物输送和组织修复。对于微创疗法，原位形成凝胶支架是可取的，但要在组织样强度和细胞大小的孔隙率之间取得平衡，仍需克服技术挑战，尤其是对于颅内和骨科疗法。在此，据报道，一种受天然丝纤维液晶纺丝工艺启发的新方法可制备具有良好的临床前功效和独特特性的可注射丝凝胶支架，包括1）用于微创手术的原位胶凝，2）可控制的孔隙率以实现高效细胞浸润和理想的降解，3）与天然软组织的模量范围兼容的弹性和可调机械性能，以及4）全水处理，避免了有毒溶剂的产生，并使得生物活性剂的装载变得容易。此外，可以实现在3D矩阵内具有可变孔隙率和生物活性剂梯度的分层结构的异质丝凝胶支架，以实现持续的药物释放和引导的组织再生。在啮齿动物模型中的临床前功效研究表明，细菌和神经胶质瘤具有有效的抑制作用，并且对骨再生和血管生成具有积极作用。可以实现在3D矩阵中具有可变孔隙率和生物活性剂梯度的分层结构异质丝绸凝胶支架，以实现持续的药物释放和引导的组织再生。在啮齿动物模型中的临床前功效研究表明，细菌和神经胶质瘤具有有效的抑制作用，并且对骨再生和血管生成具有积极作用。可以实现在3D矩阵内具有可变孔隙率和生物活性剂梯度的分层结构异质丝绸凝胶支架，以实现持续的药物释放和引导的组织再生。在啮齿动物模型中的临床前功效研究表明，细菌和神经胶质瘤具有有效的抑制作用，并且对骨再生和血管生成具有积极作用。

更新日期：2020-08-19

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