Recapture and recycling of irrigation water is often required to meet enormous water demands at horticultural nurseries. We tested four water types associated with a recycled irrigation system at a commercial container nursery in southern California for presence of oomycete plant pathogens from July 2015 to December 2017. These water types included: the main source of water originating from a reservoir, retention water from an on-site collection pond, irrigation water received by different growing areas within the nursery, and irrigation runoff captured in polyethylene sheet-lined runoff channels. The genera Phytophthora, Pythium, and Phytopythium together contributed more than 85% of the total oomycete population detected in the recycled irrigation system. The Phytophthora and Pythium genera were represented by member species from nine (1–4, 6–10) and eight (A, B, D-F, H-J) different sub-generic clades, respectively. Incoming water sourced from the reservoir was found to harbor known plant pathogens such as Phytophthora citricola-complex, P. capsici-cluster, P. tropicalis, P citrophthora-cluster, P. nemorosa-cluster, P. riparia, P. cryptogea-complex, P. parsiana-cluster, P. sp. nov. aff. kernoviae, Pythium dissotocum-complex, Py. oligandrum-cluster, Py. irregulare, and Phytopythium litorale. Runoff water showed the highest oomycete species richness and frequency of detection with both filtration and leaf baiting methods. In addition to plant pathogens, oomycete fish pathogens such as Aphanomyces laevis, Pythium chondricola-complex, Pythium flevoense-complex, and Saprolegnia diclina-complex were also detected in greater abundance in the recycled irrigation water. The oomycete species richness in the runoff water was correlated with several environmental parameters such as soil temperature. Greater oomycete richness in incoming water was associated with higher soil temperatures, whereas richness in runoff declines with increasing soil temperature, likely suggesting connections to weather-dependent nursery operations.

为了满足园艺苗圃的巨大用水需求，经常需要对灌溉用水进行回收和再循环。我们从2015年7月至2017年12月在加利福尼亚州南部的一家商业集装箱苗圃中测试了四种与循环灌溉系统相关的水类型，以检测卵菌类植物病原体的存在。这些水类型包括：来自水库的主要水源，来自水库的保留水。一个现场收集池，苗圃内不同生长区域接收的灌溉水，以及灌溉水径流收集在衬有聚乙烯薄片的径流渠道中。该属疫，腐霉和Phytopythium加在一起，在循环灌溉系统中检测到的卵菌总数超过了85％。的疫霉属和腐霉属是由构件物种从9（1-4，6-10）和8（A，B，DF，HJ）不同的子通用分支，分别表示。从贮存来源进入的水被发现怀有已知植物病原体如致病疫蚜-配合物，辣椒疫霉-cluster，P.热带 P citrophthora -cluster，P.苈-cluster，P.河岸，P.隐地-配合物，P. parsiana-群集，P. sp。十一月 aff。kernoviae，腐霉dissotocum -complex ，PY。寡聚簇，Py。不规则的，和疫霉。径流水具有最高的卵菌种丰富度，并且采用过滤和诱饵方法检出的频率最高。除了植物病原体，卵菌纲鱼类病原体如丝囊蟾，腐霉chondricola -complex，腐霉flevoense复形，和水霉回收的灌溉水中还检测到大量的复​​合物。径流水中卵菌种的丰富度与土壤温度等几个环境参数有关。进入水中的卵菌丰富度较高与土壤温度升高相关，而径流丰富度随土壤温度升高而下降，这可能暗示与依赖天气的苗圃操作有关。