Abstract This study was conducted to assess the influence of dietary supplementation of nano Zn oxide (nZnO), compared to Zn oxide (ZnO), on performance, rumen fermentation, leukocytes, total antioxidant capacity (TAC), blood serum enzymes and minerals in the ewes. Thirty pregnant Khorasan-Kurdish ewes were assigned to one of the 3 experimental groups (10 replicates) in a randomised complete block design. The ewes received 1- control diet without supplementary Zn, containing 22.4 or 24.3 mg of Zn/kg dry matter (DM) for pre- and post-partum periods, respectively, 2- diet supplemented with ZnO (30 or 40 mg of supplementary Zn/kg DM for pre- and post-partum periods, respectively) or 3- diet supplemented with nZnO (30 or 40 mg of supplementary Zn/kg DM for pre- and post-partum periods, respectively). Dry matter intake (DMI), DM digestibility (DMD), rumen parameters, milk performance and blood leukocytes of the ewes were determined. Moreover, the ewes and their suckling lambs were assessed for TAC, blood serum enzymes and minerals. Regardless of Zn form, the additional Zn increased DMD, DMI, ruminal volatile fatty acids (VFA), milk yield, TAC and blood serum Zn, but decreased milk somatic cell count (SCC) and ruminal ammonia-N (P 0.05) the TAC, aspartate aminotransferase, alanine aminotransferase, gamma-glutamyl transferase, Cu, Ca, P and Mg concentrations in the blood serum of ewes. Moreover, feeding the mother ewes with the diets containing supplementary Zn sources had no effect (P > 0.05) on the blood serum concentrations of TAC, enzymes and minerals in their suckling lambs. Overall, the use of nZnO instead of ZnO could be a useful way in improving DMI, DMD, TAC in the rumen fluid and milk, leukocytes and milk Zn concentration of the ewes, without adverse effects on the blood serum concentrations of Cu, Ca, P, Mg and Zn-dependent enzymes.

中文翻译：

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饲喂纳米氧化锌对母羊性能、瘤胃发酵、白细胞、抗氧化能力、血清酶和矿物质的影响

摘要 本研究旨在评估与氧化锌 (ZnO) 相比，日粮添加纳米氧化锌 (nZnO) 对性能、瘤胃发酵、白细胞、总抗氧化能力 (TAC)、血清酶和矿物质的影响。母羊。在随机完整区组设计中，将 30 只怀孕的呼罗珊-库尔德母羊分配到 3 个实验组之一（10 个重复）。母羊在产前和产后接受 1- 不含补充锌的对照饮食，分别含有 22.4 或 24.3 毫克 Zn/kg 干物质 (DM)，2-补充有氧化锌的饮食（30 或 40 毫克补充锌） /kg DM 分别用于产前和产后）或 3- 饮食补充 nZnO（分别为 30 或 40 mg 补充 Zn/kg DM 用于产前和产后）。干物质摄入量 (DMI)、干物质消化率 (DMD)、测定了母羊的瘤胃参数、产奶性能和血液白细胞。此外，还评估了母羊及其哺乳羔羊的 TAC、血清酶和矿物质。不管锌的形式如何，额外的锌增加了 DMD、DMI、瘤胃挥发性脂肪酸 (VFA)、牛奶产量、TAC 和血清锌，但降低了牛奶体细胞计数 (SCC) 和瘤胃氨氮 (P 0.05) TAC 、天冬氨酸氨基转移酶、丙氨酸氨基转移酶、γ-谷氨酰转移酶、母羊血清中的铜、钙、磷和镁浓度。此外，用含有补充锌源的日粮喂养母羊对其哺乳羔羊的血清 TAC、酶和矿物质浓度没有影响（P > 0.05）。总体而言，使用 nZnO 代替 ZnO 可能是改善 DMI、DMD、