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Surface plasmon resonance analysis for detecting non-structural protein 1 of dengue virus in Indonesia.
Saudi Journal of Biological Sciences Pub Date : 2020-06-17 , DOI: 10.1016/j.sjbs.2020.06.018
Widoretno 1, 2 , Agus Sjahrurachman 3 , Beti Ernawati Dewi 4 , Kenny Lischer 5, 6 , Diah Kartika Pratami 7 , Darin Flamandita 5 , Muhamad Sahlan 5, 6
Affiliation  

Dengue is an acute febrile disease caused by dengue virus (DENV) that is transmitted by Aedes sp., which causes serious health conditions in many countries. Non-structural protein 1 (NS1) is a co-factor for the RNA replication of this virus, which represents a new strategy for the identification of dengue. Prompt and accurate laboratory diagnosis of this infection is required to assist in patient triage and management, as well as prevent the spread of this infection. In the present study, we tested the potential of surface plasmon resonance (SPR) as a diagnostic tool for dengue infections. NS1 antigen protein was used as an analyte that targets anti-NS1 antibodies, with their interaction resulting in a change in the refractive index. In comparison to currently available gold-standard detection methods [enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR)], SPR showed a similar sensitivity but greater efficiency and simplicity in terms of infection detection. Out of 26 samples collected from patients with dengue in Indonesia, SPR was able to correctly identify all 16 positively infected individuals at a lower concentration and a shorter period of time compared to ELISA and RT-PCR. This study revealed that SPR is a promising tool for DENV detection and potentially other diseases as well.



中文翻译:

在印度尼西亚检测登革热病毒非结构蛋白1的表面等离子体共振分析。

登革热是由伊蚊传播的登革热病毒(DENV)引起的急性发热性疾病sp。,这会在许多国家造成严重的健康状况。非结构蛋白1(NS1)是该病毒RNA复制的辅助因子,代表了鉴定登革热的新策略。需要对此感染进行及时而准确的实验室诊断,以帮助患者分类和管理,以及防止这种感染的传播。在本研究中,我们测试了表面等离振子共振(SPR)作为登革热感染诊断工具的潜力。NS1抗原蛋白被用作靶向抗NS1抗体的分析物,它们的相互作用导致折射率发生变化。与目前可用的金标准检测方法[酶联免疫吸附测定(ELISA)和逆转录聚合酶链反应(RT-PCR)]相比,在感染检测方面,SPR显示出相似的灵敏度,但效率更高且更简单。从印度尼西亚的登革热患者那里收集的26个样本中,与ELISA和RT-PCR相比,SPR能够以较低的浓度和较短的时间正确识别所有16个阳性感染的个体。这项研究表明,SPR是用于DENV检测以及其他潜在疾病的有前途的工具。

更新日期:2020-06-17
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