Abstract New HPTLC (high-performance thin-layer chromatography) methods to evaluate major bioactive compounds in mate (Ilex paraguariensis) tea were developed. Identification of compounds was based on HPTLC-MS (high-performance thin-layer chromatography–mass spectrometry), parallel to other techniques. Quantitative evaluation of the main methylxanthines and phenolic compounds in this species was accomplished by two HPTLC methods which can be performed consecutively on one single plate. A solvent system for the separation of the identified saponins is also described for qualitative evaluations. The methods for quantification were validated in terms of: robustness; LOD and LOQ (limits of detection and quantification); repeatability; intra-day and inter-day variation; ruggedness; and accuracy. The methods have proven to be suitable for evaluation of mate tea aqueous and methanolic extracts, with the advantages of: high-throughput; reduced need of plates and application procedures; and high sensitivity – low LODs and LOQs were achieved. Aqueous extracts of nineteen mate teas of different type, geographical origin and age were evaluated in terms of methylxanthines, phenolic compounds and pH. By means of principal component analysis, distinctive characteristics between mate teas could be observed.

中文翻译：

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用于分析马黛茶中主要生物活性化合物的新 HPTLC 方法

摘要 开发了新的 HPTLC（高效薄层色谱）方法来评估马黛茶中的主要生物活性化合物。化合物的鉴定基于 HPTLC-MS（高效薄层色谱-质谱），与其他技术平行。该物种中主要甲基黄嘌呤类化合物和酚类化合物的定量评估是通过两种 HPTLC 方法完成的，这些方法可以在一个单板上连续进行。还描述了用于分离已鉴定皂苷的溶剂系统，用于定性评估。量化方法在以下方面得到验证：稳健性；LOD 和 LOQ（检测限和定量限）；可重复性；日内和日间变化；坚固性；和准确性。该方法已被证明适用于马黛茶水提物和甲醇提取物的评价，具有以下优点：高通量；减少对印版和应用程序的需求；和高灵敏度——实现了低 LOD 和 LOQ。根据甲基黄嘌呤、酚类化合物和 pH 值评估了 19 种不同类型、地理来源和年龄的马黛茶的水提取物。通过主成分分析，可以观察到马黛茶之间的独特特征。