Abstract Pseudomonas fluorescens group bacterial strains with high biofilm-forming ability were isolated from cold raw milk, identified based on molecular data, and their motility, biofilm-forming ability and proteolytic activity were characterised. After 48 h incubation at 7 °C, 57 out of 67 P. fluorescens group strains produced biofilms in vitro on polystyrene microplates. Six isolates with high-ability to produce biofilms were identified as Pseudomonas simiae, Pseudomonas veronii, Pseudomonas cedrina and three strains of P. fluorescens. They were able to produce high amounts of biofilm on stainless steel surface in tryptic soy broth and UHT milk. AdnA gene was detected in the six tested strains, but in two strains that did not show any modes of motility until 96 h of incubation at 7 °C, fliC gene was not detected. AprX gene and different levels of total and heat-resistant proteolytic activity were detected in the six tested strains.

中文翻译：

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从冷生乳中分离的高生物膜形成荧光假单胞菌群细菌菌株的鉴定、系统发育表征和蛋白水解活性定量

摘要 从冷生乳中分离出具有高生物膜形成能力的荧光假单胞菌群菌株，基于分子数据进行鉴定，并对其运动性、生物膜形成能力和蛋白水解活性进行表征。在 7°C 下孵育 48 小时后，67 个荧光假单胞菌组菌株中的 57 个在聚苯乙烯微板上体外产生了生物膜。鉴定出六株具有高产生生物膜能力的菌株，分别为模拟假单胞菌、维罗假单胞菌、雪松假单胞菌和三株荧光假单胞菌。他们能够在胰蛋白酶大豆肉汤和 UHT 牛奶的不锈钢表面上产生大量的生物膜。在 6 个测试菌株中检测到 AdnA 基因，但在 7°C 下孵育 96 小时之前未显示任何运动模式的两个菌株中，未检测到 fliC 基因。