Due to their medical importance, the number of studies on the enlightenment of the biological activities of Onosma species has been increasing over the years. In this study, besides the phytochemical composition of Onosma ambigens Lacaita (Boraginaceae), in vitro antioxidant, tyrosinase and α-amylase inhibitory activities of extracts obtained by using different solvents (ethyl acetate, methanol and water) were investigated. As a result of the spectrophotometric analysis, it was determined that methanol extract was rich in both phenolics (51.19 mg GAEs/g extract) and flavonoids (45.39 mg QEs/g extract). According to the results of liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) analysis, the major components of methanol extract were determined as rosmarinic acid (37670.55 μg/g), luteolin 7-glucoside (15756.40 μg/g), apigenin 7-glucoside (15080.34 μg/g), luteolin (2289.77 μg/g), apigenin (2240.38 μg/g), chlorogenic acid (2121.92 μg/g), vanillic acid (1179.66 μg/g), hesperidin (1038.38 μg/g), 4-hydroxybenzoic acid (961.97 μg/g) and pinoresinol (631.61μg/g). It was found that methanol extract showed remarkable activity in almost all antioxidant activity tests (except ferrous ion chelating test). The activity of this extract in the phosphomolybdenum, reducing power [cupric reducing antioxidant capacity (CUPRAC) and ferric reducing antioxidant power (FRAP)] and radical scavenging [on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)] assays were measured as 1.65, 0.95, 0.52, 1.86 and 1.45 mg/mL, respectively. Phenolic and flavonoid contents, especially chlorogenic acid, vanillic acid, luteolin 7-glucoside, hesperidin and rosmarinic acid, are thought to contribute significantly to the antioxidant activity of the extracts. In tyrosinase and α-amylase inhibitory activity tests, methanol (2.81 mg/mL) and ethyl acetate (2.64 mg/mL) extracts exhibited the best activity among the others, respectively. While it is thought that chlorogenic acid and rosmarinic acid may contribute to the tyrosinase inhibitory activity of methanol extract, it has been concluded that detailed chromatographic analyzes should be performed for detection of the compounds responsible for α-amylase inhibitory activity.

由于它们的医学重要性，多年来，对Onosma物种的生物活性的启发研究的数量一直在增加。在这项研究中，除了的植物化学物质组合物滇ambigens Lacaita（紫草科），在体外研究了使用不同溶剂（乙酸乙酯，甲醇和水）获得的提取物的抗氧化剂，酪氨酸酶和α-淀粉酶的抑制活性。通过分光光度分析的结果，确定甲醇提取物富含酚类（51.19 mg GAEs / g提取物）和类黄酮（45.39 mg QEs / g提取物）。根据液相色谱电喷雾串联质谱法（LC-ESI-MS / MS）的分析结果，确定了甲醇提取物的主要成分为迷迭香酸（37670.55μg/ g），木犀草素7-葡萄糖苷（15756.40μg/ g） ），芹菜素7-葡萄糖苷（15080.34μg/ g），木犀草素（2289.77μg/ g），芹菜素（2240.38μg/ g），绿原酸（2121.92μg/ g），香草酸（1179.66μg/ g），橙皮苷（1038.38） μg/ g），4-羟基苯甲酸（961.97μg/ g）和松脂醇（631.61μg/ g）。发现甲醇提取物在几乎所有抗氧化剂活性测试中都表现出显着的活性（亚铁离子螯合测试除外）。该提取物在磷钼中的活性，还原能力[铜还原抗氧化剂能力（CUPRAC）和三价铁还原抗氧化剂能力（FRAP）]和自由基清除作用[对1,1-二苯基-2-吡啶并肼基（DPPH）和2,2' [-叠氮基双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）]测定的分别为1.65、0.95、0.52、1.86和1.45 mg / mL。酚和类黄酮的含量，特别是绿原酸，香草酸，木犀草素7-葡糖苷，橙皮苷和迷迭香酸被认为对提取物的抗氧化活性有显着贡献。在酪氨酸酶和α-淀粉酶抑制活性测试中，甲醇（2.81 mg / mL）和乙酸乙酯（2。64 mg / mL）提取物分别表现出最好的活性。虽然认为绿原酸和迷迭香酸可能对甲醇提取物的酪氨酸酶抑制活性有贡献，但已得出结论，应进行详细的色谱分析以检测负责α-淀粉酶抑制活性的化合物。