Antibacterial and mechanical properties of arginine-containing glass ionomer cements.,Dental Materials

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Antibacterial and mechanical properties of arginine-containing glass ionomer cements.
Dental Materials ( IF 4.6 ) Pub Date : 2020-06-17 , DOI: 10.1016/j.dental.2020.05.012
Mohammed Nadeem Bijle ₁ , Manikandan Ekambaram ₂ , Edward C M Lo ₃ , Cynthia Kar Yung Yiu ₁

Affiliation

Objective

The study investigated the effect of incorporating l-arginine (Arg) in a glass ionomer cement (GIC) on its mechanical properties and antibacterial potential.

Methods

Pre-determined proportions (1%, 2%, and 4% by wt.) of Arg were incorporated in GIC powder; while GIC without Arg served as control. The flexural strength, nanohardness, surface roughness, elemental analysis using SEM-EDX (n = 6) and F/Arg/Ca/Al/Si release in deionized water for 21 days were assessed. The antibacterial potential was evaluated in a multi-species biofilm model with Streptococcus mutans, Streptococcus sanguinis, Streptococcus gordonii, and Lactobacillus acidophilus for 72 h. Real-time qPCR was used to analyse biofilm bacterial concentrations. Propidium monoazide modification of real-time qPCR was performed to quantify viable/dead bacteria. The pH, lactic acid, ADS activity, and H₂O₂ metabolism were measured. Confocal microscopy was used to investigate the biofilm bacterial live/dead cells, density, and thickness.

Results

There was no difference in flexural strength among the different groups (p > 0.05). No significant difference in nanohardness and surface roughness was observed between 4% Arg + GIC and control (p > 0.05). The 4% Arg + GIC showed significantly higher F/Arg/Al/Si release than the other groups (p < 0.05), reduced total bacterial concentration and growth inhibition of viable S. mutans and S. sanguinis (p < 0.05). Lactic acid formation for 4% Arg + GIC was significantly higher than 1% Arg + GIC (p < 0.05). The spent media pH of 4% Arg + GIC was higher than the other groups (p < 0.05), with proportionately lower ammonia and higher H₂O₂ released (p < 0.05).

Significance

Addition of 4% l-arginine in GIC enhanced its antibacterial activity via a biofilm modulatory effect for microbial homeostasis, with no detrimental effect on its mechanical properties.

中文翻译：

含精氨酸的玻璃离聚物水泥的抗菌和力学性能。

目的

该研究调查了将1-精氨酸（Arg）掺入玻璃离聚物水泥（GIC）中对其机械性能和抗菌潜能的影响。

方法

将预定比例（按重量计分别为1％，2％和4％）的Arg掺入GIC粉末中；而没有Arg的GIC作为对照。评估了去离子水中21天的抗弯强度，纳米硬度，表面粗糙度，使用SEM-EDX（n = 6）进行的元素分析以及F / Arg / Ca / Al / Si的释放。抗菌潜力在具有多物种生物膜模型中评估变异链球菌，血链球菌，链球菌戈登，和嗜酸乳杆菌72小时。实时定量PCR用于分析生物膜细菌浓度。实时qPCR进行了单叠氮化丙锭修饰，以定量活/死细菌。pH，乳酸，ADS活性和H ₂ O ₂测定新陈代谢。共聚焦显微镜用于研究生物膜细菌活/死细胞，密度和厚度。

结果

不同组之间的抗弯强度没有差异（p> 0.05）。在4％的Arg + GIC和对照组之间，没有观察到纳米硬度和表面粗糙度的显着差异（p> 0.05）。4％的Arg + GIC显示出比其他组显着更高的F / Arg / Al / Si释放（p <0.05），降低了总细菌浓度并抑制了变形链球菌和血链球菌的生长（p <0.05）。4％Arg + GIC的乳酸形成明显高于1％Arg + GIC（p <0.05）。4％Arg + GIC的废液pH值高于其他组（p <0.05），其中氨气成比例降低，而H ₂ O ₂释放量更高（p <0.05）。