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First report of gray mold of chickpea caused by Botrytis euroamericana in the USA
Crop Protection ( IF 2.8 ) Pub Date : 2020-11-01 , DOI: 10.1016/j.cropro.2020.105297
Swarnalatha Moparthi , Cecilia Peluola , Bright Agindotan , Kevin McPhee , Mary Burrows

Abstract Gray mold caused by Botrytis spp. is one of the most important diseases of chickpea. In August 2019, chickpea plants with gray mold symptoms were submitted to the Regional Pulse Crop Diagnostic Laboratory (RPCDL) at Montana State University (MSU). To identify the causal agent, leaves were surface sterilized and plated on potato dextrose agar (PDA). The fungus was off white color and did not sporulate on PDA; sclerotia were observed after 10 days of incubation. The pathogen was identified as Botrytis euroamericana by the amplification of part of the G3PDH, HSP60, and RPB2 genes and by phylogenetic analysis. Pathogenicity tests revealed that the isolate was able to cause lesions on chickpea, lentil, dry pea, and fava bean foliage.

中文翻译:

美国第一份由欧洲灰霉病菌引起的鹰嘴豆灰霉病报告

摘要 灰霉病菌引起的灰霉病。是鹰嘴豆最重要的病害之一。2019 年 8 月,具有灰霉病症状的鹰嘴豆植物被提交给蒙大拿州立大学 (MSU) 的区域豆类作物诊断实验室 (RPCDL)。为了鉴定病原体,将叶子表面消毒并铺在马铃薯葡萄糖琼脂(PDA)上。真菌为灰白色,在PDA上未形成孢子;培养10天后观察到菌核。通过部分 G3PDH、HSP60 和 RPB2 基因的扩增和系统发育分析,病原体被鉴定为 Botrytis euroamericana。致病性测试表明,该分离物能够对鹰嘴豆、扁豆、干豌豆和蚕豆的叶子造成损害。
更新日期:2020-11-01
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