Abstract In spring 2019, an anthracnose-like disease was observed on fruit in pomegranate orchards of cv. Wonderful in the Fier region of Albania. Disease prevalence in the assayed area ranged from 8 to 10%. Symptoms appeared as light to dark brown circular, sunken lesions with increasing diameter, which then merged. This study aimed to characterize the causal agent on the basis of morphology, molecular phylogeny, and pathogenicity. Micro- and macro-morphological features revealed that the disease-associated fungus belonged to Colletotrichum gloeosporioides species complex. A multi-locus molecular phylogeny using partial sequences of barcoding genes/regions (ITS, ACT, CHS-1, GAPDH, TUB2, CAL, GS, and ApMat) ascribed strain CG1 to C. gloeosporioides sensu stricto (s.s.). Pathogenicity tests, performed by inoculating healthy fruit, confirmed C. gloeosporioides s.s. as causal agent of anthracnose on pomegranate fruit in Albania.

中文翻译：

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狭义炭疽菌作为阿尔巴尼亚石榴果实炭疽病的致病因子

摘要 2019年春季，石榴园果实发生炭疽病样病害。在阿尔巴尼亚的菲尔地区很棒。检测区域的疾病流行率为 8% 至 10%。症状表现为直径逐渐增大的浅至深棕色圆形凹陷病灶，然后合并。本研究旨在根据形态学、分子系统发育和致病性来表征病原体。微观和宏观形态特征表明，与疾病相关的真菌属于Colletotrichum gloeosporioides 物种复合体。使用条形码基因/区域（ITS、ACT、CHS-1、GAPDH、TUB2、CAL、GS 和 ApMat）部分序列的多位点分子系统发育将 CG1 菌株归于狭义球孢子菌 (ss)。通过接种健康水果进行的致病性测试证实了 C.