A novel reverse transcription-based loop-mediated isothermal amplification (LAMP) strategy for miRNA detection has been developed. This method consists of two stem-loop probes inspired by the dumbbell-shaped amplicons and inner primers used in conventional LAMP reactions. Termed "terminal hairpin formation and self-priming" (THSP), this reaction incorporates phosphorothioated (PS) modifications to achieve DNA folding and extension without primers. The final signal is monitored by a sequence-specific detection probe, which minimizes the background noise. We suggest that our rapid, facile, and reliable LAMP method will be a promising candidate for detecting miRNA in biomedical applications.

中文翻译：

---

基于逆转录环介导的等温扩增策略，用于使用硫代磷探针实时检测 miRNA

已经开发了一种用于 miRNA 检测的新型基于逆转录的环介导等温扩增 (LAMP) 策略。该方法由两个茎环探针组成，其灵感来自传统 LAMP 反应中使用的哑铃形扩增子和内部引物。称为“末端发夹形成和自引发”(THSP)，该反应结合了硫代磷酸化 (PS) 修饰，无需引物即可实现 DNA 折叠和延伸。最终信号由序列特异性检测探针监测，从而最大限度地减少背景噪音。我们建议我们的快速、简便和可靠的 LAMP 方法将成为在生物医学应用中检测 miRNA 的有希望的候选者。