Abstract The effect of ethylmethylhydroxypyridine succinate (HS, Mexidol) on oocyte meiotic maturation, level of DNA damage in follicular cells, and gene expression of gremlin 1 (Grem1), hyaluronan synthase 2 (HAS2), and cyclooxygenase 2 (COX2) in cumulus cells has been investigated using a model of experimental systemic immune complex damage induced by long-term immunization of CBA mice with bovine serum albumin (BSA). According to the literature data, these genes play a critical role in oogenesis and can characterize oocyte quality. The administration of HS exhibited a protective effect on the morphological and functional state of ovarian cells. The introduction of HS attenuated the genotoxic stress induced by BSA, which was reflected in a decrease in the number of cumulus cells with severe DNA damage. The following changes in gene expression have been observed: a 1.61-fold increase in the number of COX2 mRNA and a 1.47-fold increase in Grem1 mRNA. A 1.38-fold increase in the content of HAS2 gene transcripts has also been demonstrated, although this change was not statistically significant. The observed changes had a positive effect on oocyte meiotic maturation and were accompanied by a significant increase in the oocyte number during metaphase I and II. It is proposed that HS can be successfully used in the therapy of immune complex diseases.

中文翻译：

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Ethylmethylhydroxypyridine Succinate (Mexidol) 对卵母细胞减数分裂成熟、基因组完整性和系统免疫复合物损伤条件下小鼠卵丘细胞基因表达变化的影响

摘要 乙基甲基羟基吡啶琥珀酸酯 (HS, Mexidol) 对卵母细胞减数分裂成熟、卵泡细胞 DNA 损伤水平以及卵丘细胞中 gremlin 1 (Grem1)、透明质酸合成酶 2 (HAS2) 和环氧合酶 2 (COX2) 基因表达的影响已经使用由牛血清白蛋白 (BSA) 长期免疫 CBA 小鼠诱导的实验性全身免疫复合物损伤模型进行了研究。根据文献数据，这些基因在卵子发生中起着关键作用，可以表征卵母细胞的质量。HS的施用对卵巢细胞的形态和功能状态表现出保护作用。HS 的引入减弱了 BSA 诱导的基因毒性应激，这反映在具有严重 DNA 损伤的卵丘细胞数量减少。已观察到以下基因表达的变化：COX2 mRNA 的数量增加了 1.61 倍，Grem1 mRNA 的数量增加了 1.47 倍。HAS2 基因转录本的含量也增加了 1.38 倍，尽管这种变化没有统计学意义。观察到的变化对卵母细胞减数分裂的成熟有积极影响，并伴随着中期 I 和 II 期间卵母细胞数量的显着增加。提出HS可成功用于免疫复杂疾病的治疗。观察到的变化对卵母细胞减数分裂的成熟产生积极影响，并伴随着中期 I 和 II 期间卵母细胞数量的显着增加。提出HS可成功用于免疫复杂疾病的治疗。观察到的变化对卵母细胞减数分裂的成熟产生积极影响，并伴随着中期 I 和 II 期间卵母细胞数量的显着增加。提出HS可成功用于免疫复杂疾病的治疗。