Nanoscale metal-organic frameworks (nano MOFs) have emerged as a promising biomedical nanoplatform because of their unique properties. However, the exploration of nano MOFs in photoacoustic (PA) imaging is still limited. Here, a novel hydrogen sulfide (H₂S)-activated nano copper-based MOF (Cu-MOF) was developed as a near-infrared (NIR) ratiometric PA probe for in vivo monitoring of endogenous H₂S level and orthotopic colorectal cancer imaging via in situ reaction of nano Cu-MOFs with endogenous H₂S that is closely associated with tumor growth and proliferation in colon cancer. The synthesized nano Cu-MOFs displayed excellent PA responsiveness towards tumor H₂S level with high selectivity and rapid kinetics. The result suggests the developed probe may provide a unique opportunity to investigate the malignant behaviors of H₂S-associated events in vivo.

纳米级金属有机框架（纳米MOF）因其独特的性能而成为有前途的生物医学纳米平台。但是，在光声（PA）成像中对纳米MOF的探索仍然有限。在这里，开发了一种新型的硫化氢（H ₂ S）激活的纳米铜基MOF（Cu-MOF）作为近红外（NIR）比例PA探针，用于体内监测内源性H ₂ S水平和原位结直肠癌通过纳米Cu-MOF与内源性H ₂ S的原位反应进行成像，该H ₂ S与结肠癌的肿瘤生长和增殖密切相关。合成的纳米Cu-MOFs对肿瘤H ₂表现出优异的PA反应性S级具有高选择性和快速动力学。结果表明，开发的探针可能提供一个独特的机会来研究体内H ₂ S相关事件的恶性行为。