Staphylococcal wound infections range from mild to severe with life-threatening complications. The challenge of controlling such infections is related to bacterial biofilm formation, which is a major factor contributing to antibiotic resistance and infection recurrence. In this study, four clinical isolates of staphylococci species; two isolates of methicillin-resistant Staphylococcus aureus (MRSA) and two methicillin-sensitive Staphylococcus aureus (MSSA) isolates. The identification of bacterial species based on cell morphology, initial biochemical tests, and the VITEK2 system were used to confirm the clinical microbiological diagnosis. Antibiotic sensitivity testing showed that the isolated staphylococci were highly resistant to the following antibiotics, amoxicillin, penicillin G, cefotaxime, and methicillin. Combinations of cefotaxime with the cell-free supernatants (CFS) of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895, each one separately showed complementary activity against the tested staphylococci. The co-aggregation capability of the tested bacilli as beneficial bacteria against isolated staphylococci was also evaluated. The data showed a strong co-aggregation with scores (+ 3, + 4) which were reported between the bacilli strains and the isolated staphylococci. Furthermore, the CFS of bacilli strains showed an inhibitory effect against biofilm-associated MRSA and MSSA. These findings confirmed the ability of beneficial bacteria to compete with the pathogens at the site of colonization or for the source of nutrients and, eventually, lead to inhibition of the pathogens’ capability of causing a wound infection. Such beneficial bacteria could play an important role in future pharmaceutical and industrial applications.

葡萄球菌伤口感染范围从轻度到严重，并危及生命。控制此类感染的挑战与细菌生物膜形成有关，细菌生物膜形成是导致抗生素耐药性和感染复发的主要因素。在这项研究中，有四种临床分离的葡萄球菌。耐甲氧西林的两种分离的金黄色葡萄球菌（MRSA）和两个甲氧西林敏感的金黄色葡萄球菌（MSSA）分离株。根据细胞形态，初始生化测试和VITEK2系统对细菌种类进行鉴定，以确认临床微生物学诊断。抗生素敏感性测试表明，分离出的葡萄球菌对下列抗生素具有高度抗药性，其中包括阿莫西林，青霉素G，头孢噻肟和甲氧西林。头孢噻肟与枯草芽孢杆菌KATMIRA1933和解淀粉芽孢杆菌的无细胞上清液（CFS）的组合B-1895，每一个分别显示出针对所测试葡萄球菌的互补活性。还评估了测试细菌作为有益细菌对抗分离的葡萄球菌的共聚集能力。数据显示，细菌菌株和分离的葡萄球菌之间有很强的共计得分（+ 3，+ 4）。此外，细菌菌株的CFS显示出对与生物膜相关的MRSA和MSSA的抑制作用。这些发现证实了有益细菌与病原体在定殖部位或营养源竞争的能力，并最终导致对病原体引起伤口感染的能力的抑制。这些有益细菌可能在未来的制药和工业应用中发挥重要作用。