A high-resolution melting analysis method for the effective discrimination of three Sepia species,Journal of Consumer Protection and Food Safety

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A high-resolution melting analysis method for the effective discrimination of three Sepia species
Journal of Consumer Protection and Food Safety ( IF 1.4 ) Pub Date : 2020-06-16 , DOI: 10.1007/s00003-020-01289-8
Yun Zhao , Weiwei Song , Ronghua Li , Chunlin Wang , Changkao Mu

A high resolution melting (HRM) curve analysis of single nucleotide polymorphisms (SNPs) was used to identify three Sepia species, commonly found on the south coast of China. The mitochondrial 16S rRNA gene sequences from Sepiella japonica, Sepia pharaonis and Sepia lycidas were amplified and aligned. A total of 19 SNPs and seven deletion sites were identified in 122-base-pair (bp) amplicons. All samples from the three Sepia species were clustered into three curves based on HRM analysis, in line with the sequencing results. This new method thus enables a quick, economical and efficient identification of Sepia species.

更新日期：2020-06-16

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