The virome of babaco (Vasconcellea x heilbornii) —a non-traditional fruit crop native to Ecuador— was investigated by high-throughput sequencing (HTS) on plants obtained from a commercial nursery. Six virus-like sequences were detected, including the full length of papaya ringspot virus (PRSV) and an RNA-dependent-RNA-polymerase (RdRp) sequence with homology to papaya virus Q. Three RNA sequences were found with homology, respectively, to apple latent spherical virus (genus Cheravirus, 71% nt identity), cherry leaf roll virus (genus Nepovirus, 54% nt identity) and Citrullus lanatus cryptic virus (genus Deltapartitivirus, 66% nt identity); whereas a DNA pararetrovirus-like sequence with homology to citrus endogenous pararetrovirus (58% nt identity) was also detected. RT-PCR-based virus surveys on a total of 284 samples collected from three provinces revealed that the partitivirus- and pararetrovirus-like sequences were present in 100% of tested plants; whereas the other virus sequences were detected in up to 68% of plants and were associated with different symptoms. This work provides information on the occurrence and prevalence of PRSV and five additional virus-like sequences in babaco, a vegetatively propagated crop, supporting the need for a virus-free certification program.

通过高通量测序（HTS），对从商业苗圃获得的植物进行了babaco（Vasconcellea x heilbornii）病毒（厄瓜多尔本土的非传统水果作物）的研究。检测到六种病毒样序列，包括全长木瓜环斑病毒（PRSV）和与木瓜病毒Q具有同源性的RNA依赖性RNA聚合酶（RdRp）序列。发现了三个与RNA同源的RNA序列苹果潜球形病毒（属Cheravirus， 71％NT同一性），樱桃卷叶病毒（属Nepovirus，54％核苷酸同一性）和西瓜隐蔽病毒（属Deltapartitivirus，66％的nt身份）；而还检测到了与柑橘内源性副逆转录病毒具有同源性的DNA副逆转录病毒样序列（58％nt相同）。对来自三个省的总共284个样本进行的基于RT-PCR的病毒调查表明，在100％的受试植物中存在类似partitivirus和pararetrovirus的序列。而其他病毒序列最多可在68％的植物中检测到，并具有不同的症状。这项工作提供了有关无性繁殖的作物babaco中PRSV和5种其他病毒样序列的发生和流行的信息，支持了无病毒认证计划的需要。