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A ratiometric photoacoustic imaging approach for semi-quantitative determination of aggregation efficiency in vivo.
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-16 , DOI: 10.1039/d0nr03218b Bo Peng 1 , Xiu-Mei Liu , Hsian-Rong Tseng , Li-Li Li , Hao Wang
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-16 , DOI: 10.1039/d0nr03218b Bo Peng 1 , Xiu-Mei Liu , Hsian-Rong Tseng , Li-Li Li , Hao Wang
Affiliation
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In vivo self-assembly not only endows dynamic supramolecules with various biological functions, but also realizes metabolic differences, and improves the level of diagnosis and treatment. However, the method of measuring aggregation efficiency in vivo is still challenging. In this work, we first proposed a ratiometric photoacoustic imaging method to measure the aggregation efficiency of molecules in vivo in real time and semi-quantitatively. Similar to the traditional fluorescence method, the ratiometric photoacoustic signal has a typical exponential relationship with the aggregation efficiency, which is defined as the percentage of aggregation molecules in the total molecules. Then, we proposed a ratiometric photoacoustic (PA) probe, which can be tailored by cathepsin E and self-assembled into nanofibers in situ inside pancreatic cancer cells. The maximum aggregation efficiency of 10−5 M PA probe was 58% after 2 hours of incubation. After intratumoral administration in xenografted pancreatic tumor mice, the highest aggregation efficiency was found to be 36% 6 hours after the injection. The ratiometric PA probe provides us with a real-time method to detect the aggregation efficiency in vivo, which is helpful to deepen the understanding of the dynamic assembly process and optimize the design of supramolecules.
中文翻译：
一种用于半定量确定体内聚集效率的比率光声成像方法。
体内自组装不仅赋予动态超分子以多种生物学功能，而且实现了新陈代谢的差异，提高了诊断和治疗水平。然而，测量体内聚集效率的方法仍然具有挑战性。在这项工作中，我们首先提出了一种比例光声成像方法来测量体内分子的聚集效率。实时和半定量。与传统的荧光方法类似，比例光声信号与聚集效率具有典型的指数关系，聚集效率定义为聚集分子在总分子中的百分比。然后，我们提出了一种比例光探测器（PA），可以通过组织蛋白酶E对其进行定制，并在胰腺癌细胞内原位自组装成纳米纤维。孵育2小时后，10 -5 M PA探针的最大聚集效率为58％。在异种移植的胰腺肿瘤小鼠中进行肿瘤内给药后，发现最高的聚集效率是注射后6小时的36％。比率式PA探针为我们提供了一种实时方法来检测聚集效率体内，这有助于加深对动态组装过程的了解并优化超分子的设计。
更新日期：2020-06-16
中文翻译：
一种用于半定量确定体内聚集效率的比率光声成像方法。
体内自组装不仅赋予动态超分子以多种生物学功能，而且实现了新陈代谢的差异，提高了诊断和治疗水平。然而，测量体内聚集效率的方法仍然具有挑战性。在这项工作中，我们首先提出了一种比例光声成像方法来测量体内分子的聚集效率。实时和半定量。与传统的荧光方法类似，比例光声信号与聚集效率具有典型的指数关系，聚集效率定义为聚集分子在总分子中的百分比。然后，我们提出了一种比例光探测器（PA），可以通过组织蛋白酶E对其进行定制，并在胰腺癌细胞内原位自组装成纳米纤维。孵育2小时后，10 -5 M PA探针的最大聚集效率为58％。在异种移植的胰腺肿瘤小鼠中进行肿瘤内给药后，发现最高的聚集效率是注射后6小时的36％。比率式PA探针为我们提供了一种实时方法来检测聚集效率体内，这有助于加深对动态组装过程的了解并优化超分子的设计。
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