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Magnetic nanoparticles for the measurement of cell mechanics using force-induced remnant magnetization spectroscopy.
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-16 , DOI: 10.1039/d0nr01421d Min Xu 1 , Xueyan Feng 1 , Feng Feng 1 , Hantao Pei 2 , Ruping Liu 2 , Qilong Li 1 , Chanchan Yu 1 , Di Zhang 1 , Xiuyu Wang 1 , Li Yao 1
Nanoscale ( IF 5.8 ) Pub Date : 2020-06-16 , DOI: 10.1039/d0nr01421d Min Xu 1 , Xueyan Feng 1 , Feng Feng 1 , Hantao Pei 2 , Ruping Liu 2 , Qilong Li 1 , Chanchan Yu 1 , Di Zhang 1 , Xiuyu Wang 1 , Li Yao 1
Affiliation
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Cell mechanics is a crucial indicator of cell function and health, controlling important biological activities such as cell adhesion, migration, and differentiation, wound healing, and tissue integrity. Particularly, the adhesion of cancer cells to the extracellular matrix significantly contributes to cancer progression and metastasis. Here we develop magnetic nanoparticle-based force-induced remnant magnetization spectroscopy (FIRMS) as a novel method to measure cell adhesion force. Before FIRMS experiments, interactions of magnetic nanoparticles (MNPs) with cells were investigated from a cell mechanics perspective. Subsequently adhesion force for three commonly used cancer cell lines was quantified by FIRMS. Our results indicated that the application of MNPs produced indistinguishable effects on cell viability and cell mechanical properties under experimental conditions for the FIRMS method. Then cell adhesion force was obtained, which provides force information on different cancer cell types. Our work demonstrates that MNP-based FIRMS can be applied to probe cell adhesion force and offer an alternate means for understanding cell mechanics.
中文翻译：
磁性纳米粒子，用于使用力感应式剩余磁化光谱技术测量细胞力学。
细胞力学是细胞功能和健康的关键指标，它控制着重要的生物学活动，例如细胞粘附，迁移和分化，伤口愈合以及组织完整性。特别地，癌细胞对细胞外基质的粘附显着促进了癌症的进展和转移。在这里，我们开发基于磁性纳米粒子的力感应残余磁化光谱（FIRMS）作为测量细胞粘附力的新方法。在FIRMS实验之前，从细胞力学的角度研究了磁性纳米粒子（MNP）与细胞的相互作用。随后通过FIRMS量化了三种常用癌细胞系的粘附力。我们的结果表明，在FIRMS方法的实验条件下，MNP的应用对细胞活力和细胞力学性能产生了难以区分的影响。然后获得细胞粘附力，其提供了关于不同癌细胞类型的力信息。我们的工作表明，基于MNP的FIRMS可用于探测细胞粘附力，并为理解细胞力学提供了另一种方法。
更新日期：2020-07-16
中文翻译：
磁性纳米粒子，用于使用力感应式剩余磁化光谱技术测量细胞力学。
细胞力学是细胞功能和健康的关键指标，它控制着重要的生物学活动，例如细胞粘附，迁移和分化，伤口愈合以及组织完整性。特别地，癌细胞对细胞外基质的粘附显着促进了癌症的进展和转移。在这里，我们开发基于磁性纳米粒子的力感应残余磁化光谱（FIRMS）作为测量细胞粘附力的新方法。在FIRMS实验之前，从细胞力学的角度研究了磁性纳米粒子（MNP）与细胞的相互作用。随后通过FIRMS量化了三种常用癌细胞系的粘附力。我们的结果表明，在FIRMS方法的实验条件下，MNP的应用对细胞活力和细胞力学性能产生了难以区分的影响。然后获得细胞粘附力，其提供了关于不同癌细胞类型的力信息。我们的工作表明，基于MNP的FIRMS可用于探测细胞粘附力，并为理解细胞力学提供了另一种方法。
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