We present an optical scientist vision for a photonic platform for real-time polymerase chain reaction (qPCR) and reverse-transcription qPCR (qRT-PCR) that integrates a rapid-cycle fiber-optic PCR chamber with feedback-controlled laser heating, online fluorometric detection of PCR products, and all-optical thermometry using an optical readout from color centers of diamond nanoparticles. We show that, with a careful optimization of laser-induced heating and heat-removal geometry, this approach can radically shorten the qPCR thermal cycle relative to standard PCR systems, paving the way toward credit-card-size testing kits for fast point-of-care infection diagnosis. Faster photonic qPCR/qRT-PCR will enable a rapid and highly specific early detection of infections, including new viruses, such as SARS-CoV-2 at very early stages-in most cases, even before the onset of disease, providing much-needed lead time for early treatment and disease spread prevention.

中文翻译：

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用于快速实时聚合酶链反应的光子工具箱

我们为实时聚合酶链反应 (qPCR) 和逆转录 qPCR (qRT-PCR) 的光子平台提出了一个光学科学家的愿景，该平台将快速循环光纤 PCR 室与反馈控制的激光加热、在线荧光测量集成在一起。 PCR 产物的检测，以及使用金刚石纳米颗粒色心的光学读数的全光测温。我们表明，通过仔细优化激光诱导加热和散热几何形状，这种方法可以从根本上缩短 qPCR 热循环相对于标准 PCR 系统，为信用卡大小的快速检测试剂盒铺平了道路-护理感染诊断。更快的光子 qPCR/qRT-PCR 将能够快速和高度特异性地早期检测感染，包括在非常早期的新病毒，例如 SARS-CoV-2——在大多数情况下，