Peel is one of the major by-products in fruit processing industry. Fruit products (non-edible parts) are also considered as waste products and often discarded in the environment. Fruit peels are now serving as one of the primary sources for isolation and extraction of secondary metabolites in pharmaceutical industry. The present investigation was carried out to screen the phytochemical constituents and HPTLC analysis of peel extracts of Punica granatum and Citrus aurantifolia and their antidiabetic potential in alloxan-induced diabetic rats. Among the different solvent extracts, methanol solvent extract was found to possess more amounts of secondary metabolites. In addition, HPTLC analysis of the plant samples revealed the presence of 13 peaks in both the plants by using gallic acid as marker. Different biochemical parameters such as blood glucose, cholesterol, protein, urea, creatinine, and triglycerides level were subjected for estimation by collecting the blood samples from the treated diabetic rats after 21 days. A sharp decline in blood glucose, cholesterol, triglycerides, creatinine, and urea level was noticed when methanolic extracts of Punica granatum and Citrus aurantifolia were given to experimental animals when compared with negative control. However, protein and weight of the animal were found to be enhanced when treated with methanolic extracts of both the plants. It can be concluded that fruit peels of both the plants exhibited antidiabetic potential on alloxan-induced diabetic rats which can be attributed to wide range of active pool of secondary metabolites. Further, screening and isolation of secondary metabolites along with their mode of action is required for effective use of plant-based drugs as antihyperglycemic agent.

中文翻译：

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石榴皮和枳壳提取物对四氧嘧啶诱导的糖尿病大鼠的影响

果皮是水果加工业的主要副产品之一。水果产品（不可食用部分）也被视为废品，经常被丢弃在环境中。果皮现在是制药工业中分离和提取次级代谢产物的主要来源之一。本研究旨在筛选石榴皮和枳实的果皮提取物的植物化学成分和 HPTLC 分析及其对四氧嘧啶诱导的糖尿病大鼠的抗糖尿病潜力。在不同的溶剂提取物中，发现甲醇溶剂提取物具有更多的次级代谢物。此外，植物样品的 HPTLC 分析显示，通过使用没食子酸作为标记，两种植物中均存在 13 个峰。不同的生化参数，如血糖、胆固醇、蛋白质、尿素、肌酐和甘油三酯水平通过在 21 天后从接受治疗的糖尿病大鼠收集血样进行评估。与阴性对照相比，当将石榴和枳实的甲醇提取物给予实验动物时，注意到血糖、胆固醇、甘油三酯、肌酐和尿素水平急剧下降。然而，当用这两种植物的甲醇提取物处理时，发现动物的蛋白质和体重增加。可以得出结论，这两种植物的果皮对四氧嘧啶诱导的糖尿病大鼠表现出抗糖尿病潜力，这可归因于次级代谢产物的广泛活性池。更多，