Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Performance of commercially available anti-HDV enzyme-linked immunosorbent assays in Taiwan.
Virology Journal ( IF 4.8 ) Pub Date : 2020-06-16 , DOI: 10.1186/s12985-020-01355-5 Guan-Yu Lin , Yi-Le Wu , Cheng-Si Wang , Chia-Yun Ko , Chien-Hung Chen , Pei-Jer Chen , Po-Hsin Peng , Chao-Wei Hsu
Virology Journal ( IF 4.8 ) Pub Date : 2020-06-16 , DOI: 10.1186/s12985-020-01355-5 Guan-Yu Lin , Yi-Le Wu , Cheng-Si Wang , Chia-Yun Ko , Chien-Hung Chen , Pei-Jer Chen , Po-Hsin Peng , Chao-Wei Hsu
Hepatitis D virus (HDV) infection is a major global health issue around the world. There are approximately 15–20 million individuals infected with HDV worldwide. HDV infection usually causes increased mortality compared with infection with hepatitis B virus (HBV) alone. However, testing for the detection of HDV is not widely available in Taiwan. Therefore, the General Biologicals Corporation (GB) HDV Ab kit was developed for detecting anti-HDV antibodies. A total of 913 serum and 462 EDTA-treated plasma samples were obtained from HBsAg-positive individuals in three hospitals in Taiwan from June 2014 to November 2017. We used three commercially available ELISA kits, DiaPro HDV Ab, DiaSorin ETI-AB-DELTAK-2 and GB HDV Ab, which were utilized strictly according to the instructions of the manufacturers. A comparative study of the results from the GB HDV Ab kit and the other commercial ELISA kits (DiaPro and DiaSorin) was performed to determine their efficacy for anti-HDV detection. The results indicated that the sensitivity of the GB HDV Ab kit for serum and EDTA samples was 100% compared to that of the DiaPro and DiaSorin kits, whereas the specificity for serum and EDTA samples was 99.3 and 98.1%, respectively. In addition, the overall agreement of the results of the GB HDV Ab kit for the serum and EDTA samples was 99.3 and 98.3%, respectively. It is worth noting that the performance of the GB HDV Ab kit was not affected by interference from triglyceride, bilirubin, hemoglobin, or human anti-mouse antibody. The limit of detection of the GB HDV Ab kit is approximately 100-fold lower than that of the other two commercial kits. The GB HDV Ab kit, which presented equivalent sensitivity and specificity compared to both certified anti-HDV kits, would be a suitable kit for HDV diagnosis in Taiwan.
更新日期：2020-06-16
京公网安备 11010802027423号