Little is known about the distribution and phylogeny of bacterial endosymbionts in oribatid mites (Acari: Oribatida). Thus, we undertook the issue of occurrence of these microbial symbionts in this arthropod group. We used PCR technique for detection of Wolbachia in Damaeus onustus. Phylogenetic analysis of the bacterium was conducted based on the 16S rDNA sequence. To the best of our knowledge, we present a novel finding of Wolbachia infection in the sexually reproducing oribatid mite, D. onustus. The presence of uninfected individuals (ca. 93%) suggests that the bacteria do not function as primary symbionts. A comparison of the bacterial 710-bp 16S rDNA sequence detected in the oribatid mite with the sequences deposited in GenBank revealed its 92–93% similarity to the 16S rDNA sequences of Wolbachia identified in some springtails (Collembola) and Bryobia sp. mite. Bacteria from D. onustus showed phylogenetic relationships with Wolbachia from springtails, Megalothorax minimus and Neelus murinus, which were included by other authors into a separate Wolbachia clade. Our finding suggests that the strains of Wolbachia from D. onustus may form a new Wolbachia supergroup.

中文翻译：

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第一份报告沃尔巴克氏体在Damaeus onustus（蜱螨亚纲：甲螨）

关于细菌内共生菌在oribatid螨中的分布和系统发育知之甚少（Acari：Oribatida）。因此，我们研究了该节肢动物群中这些微生物共生菌的发生问题。我们使用PCR技术检测了Dauaeus onustus中的Wolbachia。根据16S rDNA序列进行细菌的系统发育分析。据我们所知，我们提出了在有性生殖的蝙蝠螨D. onustus中出现Wolbachia感染的新发现。未感染个体（约93％）的存在表明该细菌不能作为主要的共生体起作用。将在oribatid螨中检测到的细菌710 bp 16S rDNA序列与GenBank中保存的序列进行比较，发现其与某些跳尾（Collembola）和苔藓菌属中鉴定的Wolbachia的16S rDNA序列有92–93％的相似性。螨。D. onustus的细菌显示与跳虫，Megalothorax minimus和Neelus murinus的Wolbachia发生系统发育关系，其他作者将其包括在单独的Wolbachia进化枝中。我们的发现表明，来自D. onustus的Wolbachia菌株可能形成一个新的Wolbachia超群。