A label-free electrochemical DNA biosensor for kanamycin detection based on diblock DNA with poly-cytosine as a high affinity anchor on graphene oxide.,Analytical Methods

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A label-free electrochemical DNA biosensor for kanamycin detection based on diblock DNA with poly-cytosine as a high affinity anchor on graphene oxide.
Analytical Methods ( IF 2.7 ) Pub Date : 2020-06-16 , DOI: 10.1039/d0ay00025f
Xiaoyan He ₁ , Huimin Han ₁ , Wenyu Shi ₁ , Jiandi Dong ₁ , Xiong Lu ₁ , Wu Yang ₁ , Xiaoquan Lu ₁

Affiliation

It is urgent to develop a more simple and sensitive method to detect antibiotic residues considering the harm of antibiotic residues in food to the human body. Herein we designed a label-free electrochemical DNA biosensor for the sensitive detection of kanamycin (KAN) based on diblock DNA with a 15-mer of poly-cytosine (poly-C). The diblock DNA can be immobilized on graphene oxide (GO) due to strong physical adsorption between the 15-mer of poly-C and GO. The aptamer of KAN acted as the other block for rapidly binding the target. It can specifically capture the target, which leads to the change of electrochemical signal. Consequently, the DNA biosensor exhibited high sensitivity and specificity towards KAN, the linear range was from 0.05 pM to 100 nM with a detection limit of 0.0476 pM. The developed DNA biosensor was constructed easily and showed promising applications for the detection of antibiotic residues for food safety.

中文翻译：

一种无标记的电化学DNA生物传感器，用于检测卡那霉素，该传感器基于二嵌段DNA，多胞嘧啶作为氧化石墨烯上的高亲和力锚。

考虑到食品中抗生素残留对人体的危害，迫切需要开发一种更简单，更灵敏的方法来检测抗生素残留。本文中，我们设计了一种无标记的电化学DNA生物传感器，用于基于含15个聚胞嘧啶（poly-C）的二嵌段DNA灵敏检测卡那霉素（KAN）。由于在poly-C和GO的15-mer之间有很强的物理吸附作用，二嵌段DNA可以固定在氧化石墨烯（GO）上。KAN的适体充当了快速结合靶标的另一个障碍。它可以特异性捕获靶标，从而导致电化学信号的变化。因此，该DNA生物传感器对KAN表现出高灵敏度和特异性，线性范围为0.05pM至100nM，检测极限为0.0476pM。

更新日期：2020-07-16

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