Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues.,Journal of Agricultural and Food Chemistry

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Changes in the Proanthocyanidin Composition and Related Gene Expression in Bilberry (Vaccinium myrtillus L.) Tissues.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-06-16 , DOI: 10.1021/acs.jafc.0c02158
Jussi Suvanto ₁ , Katja Karppinen ₂ , Kaisu Riihinen ₃ , Laura Jaakola _{2,

4} , Juha-Pekka Salminen ₁

Affiliation

Berries of genus Vaccinium are rich in flavonoids and proanthocyanidins (PAs). We studied the PA composition and biosynthesis in bilberry (Vaccinium myrtillus L.) tissues and during fruit development. Soluble PAs, analyzed by UHPLC–MS/MS, were most abundant in stem and rhizome with the mean PA polymerization level varying between 4 and 6 in all tissues. Both A- and B-type PAs were present in all tissues. Procyanidin subunits were more common than prodelphinidin subunits in PAs. During fruit ripening, the amount of procyanidin subunits decreased while prodelphinidin subunits and F3′5′H expression increased, indicating a shift in biosynthesis toward the delphinidin branch of the flavonoid pathway. Epicatechin was the most abundant flavan-3-ol in all tissues. Expression of ANR and three isolated LAR genes, analyzed by qRT-PCR, showed connection to accumulation of PAs and flavan-3-ols biosynthesized from different flavonoid branches. Insoluble PAs accumulated during berry development, suggesting that PAs are not recycled after biosynthesis.

中文翻译：

越桔组织中原花青素组成和相关基因表达的变化。

属浆果越橘含有丰富的类黄酮和原花青素（PAS）。我们研究了越桔（Vaccinium myrtillus L.）组织和果实发育过程中PA的组成和生物合成。通过UHPLC-MS / MS分析，可溶性PA在茎和根茎中含量最高，在所有组织中平均PA聚合水平在4至6之间变化。在所有组织中均存在A型和B型PA。在PA中，原花青素亚基比原花青素亚基更常见。在果实成熟期间，原花青素亚基的数量减少，而原花青素亚基和F3'5'H的表达增加，表明生物合成向类黄酮途径的delphinidin分支转移。表儿茶素是所有组织中最丰富的黄烷-3-醇。表达通过qRT-PCR分析的ANR和三个分离的LAR基因显示与从不同类黄酮分支生物合成的PA和flavan-3-ol的积累有关。浆果发育过程中积累了不溶性PA，这表明PA在生物合成后不会被回收。

更新日期：2020-07-15

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