ABSTRACT Histone deacetylases (HDACs) play an important role in plant stress response. In Brachypodium distachyon, which is model species for molecular biology research on monocot plants, the histone deacetylase BdHD1, homologous to AtHDAC1 of the RPD3/HDA1 class, functions as a positive regulator in the plant drought stress response. AtHDAC1 has been found to interact with transcription factors to regulate gene expression. However, the drought-responsive transcription factors that interact with BdHD1 have not been identified yet. Previously, we identified BdWRKY24 and BdMYB22 as drought responsive transcription factors in Brachypodium. In this study, we used yeast two-hybrid (Y2 H) and bimolecular fluorescence complementation (BiFC) assays to show that BdHD1 interacts with BdWRKY24 and BdMYB22. Our findings provides a base to investigate BdHD1-transcription factor complexes in the context of drought stress response in Brachypodium.

中文翻译：

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BdHD1 是二穗短柄草的一种组蛋白脱乙酰酶，与两个干旱响应转录因子 BdWRKY24 和 BdMYB22 相互作用

摘要 组蛋白脱乙酰酶（HDAC）在植物逆境响应中发挥重要作用。在单子叶植物分子生物学研究的模型物种二穗短柄草中，组蛋白脱乙酰酶 BdHD1 与 RPD3/HDA1 类的 AtHDAC1 同源，在植物干旱胁迫反应中发挥正向调节作用。AtHDAC1 已被发现与转录因子相互作用来调节基因表达。然而，与 BdHD1 相互作用的干旱响应转录因子尚未确定。此前，我们将 BdWRKY24 和 BdMYB22 鉴定为短柄草中的干旱响应转录因子。在这项研究中，我们使用酵母双杂交 (Y2 H) 和双分子荧光互补 (BiFC) 检测来证明 BdHD1 与 BdWRKY24 和 BdMYB22 相互作用。我们的研究结果为研究短柄草干旱胁迫反应背景下的 BdHD1 转录因子复合物奠定了基础。