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Escherichia coli as a carrier of tetracyclines and penicillins resistance in wild pheasant (Phasianus colchicus).
Journal of Environmental Science and Health, Part A ( IF 1.9 ) Pub Date : 2020-06-15 , DOI: 10.1080/10934529.2020.1777050
Lukáš Hleba 1 , Miroslava Hlebová 2 , Anton Kováčik 3 , Peter Šmehýl 4 , Nikola Hricáková 1 , Jana Petrová 1 , Mohammad Ali Shariati 5 , Juraj Čuboň 6
Affiliation  

Wild animals like pheasant seem to be a good source of information about human activities. Therefore, the wild pheasants and relative stable appendix microcenosis were selected for antibiotic resistance testing. Penicillin resistance by MALDI-TOF Mass Spectrometry and tetracyclines resistance by genetic methods using specific primers were tested. Differences between tetracycline and penicillin resistance were detected. Results showed high prevalence of resistant Escherichia coli isolated from wild pheasant appendix. E. coli isolated from wild pheasant appendix carried plasmids for penicillins and tetracyclines resistance where they were responsible for enzymatic degradation of penicillin and carried genes for regulating efflux pumps for tetracyclines. Results showed that tetracyclines and penicillins resistance is widespread between wild pheasants with a carrier as Escherichia coli isolated from relative stable microcenosis of appendix.



中文翻译:

大肠杆菌在野鸡(Phasianus colchicus)中作为四环素和青霉素抗性的载体。

野鸡等野生动物似乎是人类活动信息的良好来源。因此,选择野鸡和相对稳定的阑尾微中心菌进行抗生素耐药性测试。使用MALDI-TOF质谱对青霉素进行了抗药性测试,通过遗传方法使用特定引物对四环素进行了抗药性测试。检测到四环素和青霉素耐药性之间存在差异。结果表明,从野生野鸡附录分离出的抗性大流行。大肠杆菌从野生野鸡附录中分离得到的青霉素和四环素抗性质粒,负责青霉素的酶促降解,并调节四环素的外排泵基因。结果表明,四环素和青霉素抗性在带有载体的野生野鸡之间广泛存在,所述载体是从相对稳定的阑尾微菌落分离出来的大肠杆菌

更新日期:2020-06-15
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