Abstract

The aquaculture industry has advanced toward sustainable recirculating systems, in where parameters of food quality are strictly monitored. Despite that, as in the case of conventional aquaculture practices, the recirculating systems also suffer threats from Aeromonas spp., Vibrio spp., Streptococcus spp., among other foodborne pathogens infecting farmed fish. The aquaculture pathogens are routinely detected by conventional PCR methods or antibody-based tests, with the detection protocols confined to laboratory use. Emerging assay technologies and biosensors recently reported in the literature open new opportunities to the development of sensitive, specific, and portable analytical devices to use in the field. Techniques of DNA/RNA analysis, immunoassays and other nanomolecular technologies have been facing important advances in response time, sensitivity, and enhanced power of discrimination among and within species. Moreover, the recent developments of electrochemical and optical signal transduction have facilitated the incorporation of the innovative assays to practical miniaturized devices. In this work, it is provided a critical review over foodborne pathogen detection by existing and promising methods and biosensors applied to fish samples and extended to other food matrices. While isothermal DNA/RNA amplification methods can be highlighted among the assay methods for their promising analytical performance and suitability for point-of-care testing, the electrochemical transduction provides a way to achieve cost-effective biosensors amenable to use in the aquaculture field. The adoption of new methods and biosensors would constitute a step forward in securing sustainable aquaculture systems.

摘要

水产养殖业已朝着可持续的循环系统发展，其中严格监控食品质量参数。尽管如此，与常规水产养殖做法一样，再循环系统也受到了气单胞菌，弧菌，链球菌的威胁。spp。，以及其他感染养殖鱼类的食源性病原体。水产养殖病原体通常通过常规PCR方法或基于抗体的检测方法进行检测，检测方案仅限于实验室使用。最近在文献中报道的新兴测定技术和生物传感器为在现场使用的灵敏，特异和便携式分析设备的开发提供了新的机会。DNA / RNA分析技术，免疫测定法和其他纳米分子技术在响应时间，灵敏度以及增强物种间和物种内辨别能力方面都面临着重要的进步。而且，电化学和光信号转导的最新发展促进了将创新的测定法结合到实用的小型化装置中。在这项工作中，它通过现有和有前途的方法以及应用于鱼类样品并扩展到其他食品基质的生物传感器，对食源性病原体的检测提供了重要的评论。尽管等温DNA / RNA扩增方法因其有前途的分析性能和即时检验的适用性而在分析方法中得以突出，但电化学转导提供了一种方法，可实现适用于水产养殖领域的具有成本效益的生物传感器。采用新方法和生物传感器将构成确保可持续水产养殖系统向前迈出的一步。尽管等温DNA / RNA扩增方法因其有前途的分析性能和即时检验的适用性而在分析方法中得以突出，但电化学转导提供了一种方法，可实现适用于水产养殖领域的具有成本效益的生物传感器。采用新方法和生物传感器将构成确保可持续水产养殖系统向前迈出的一步。尽管等温DNA / RNA扩增方法因其有前途的分析性能和即时检验的适用性而在分析方法中得以突出，但电化学转导提供了一种方法，可实现适用于水产养殖领域的具有成本效益的生物传感器。采用新方法和生物传感器将构成确保可持续水产养殖系统向前迈出的一步。