The fertilization capacity of sex‐sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex‐sorted bull sperm. In this study, the washing medium and fertilization medium of sex‐sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10^–3, 1 × 10^–4, 1 × 10^–5, 1 × 10^–6 M) or Lyc (0, 1 × 10^–4, 1 × 10^–5, 1 × 10^–6, 1 × 10^–7). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex‐sorted sperm were investigated. For the sex‐sorted sperm of bulls A, B and C, 1 × 10^–3 M VC or 1 × 10^–4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10^–4 M Lyc significantly improved the blastocyst rates and their IFN‐tau expression of bulls A and C. In conclusion, supplementation of 1 × 10^–3 M VC or 1 × 10^–4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex‐sorted bull sperm during IVF procedure.

中文翻译：

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维生素C和番茄红素通过抑制氧化应激对性别排序的公牛精子的体外受精能力的保护作用。

按性别排序的精子的受精能力严重下降，这阻碍了其广泛应用。但是，关于维生素C（VC）和番茄红素（Lyc）对按性别分选的公牛精子的受精能力的影响，仍然缺乏信息。在这项研究中，对来自三个公牛个体的按性别排序的精子的洗涤培养基和受精培养基补充了不同浓度的VC（0，1×10 ^–3，1 ×10 ^–4，1 ×10 ^–5，1 ×10 ^–6  M）或Lyc（0，1×10 ^–4，1 ×10 ^–5，1 ×10 ^–6，1 ×10 ^–7）。洗涤两次并孵育1.5小时后，研究了按性别分类的精子的丙二醛（MDA）水平，磷脂酰丝氨酸（PS）易位，膜电位（Δψm）和IVF（体外受精）能力。对于公牛A，B和C的按性别分类的精子， IVF后1×10 ^–3  M VC或1×10 ^–4 M Lyc处理显着降低了MDA水平和PS易位，并增加了Δψm水平和卵裂率。当考虑胚泡时，1×10 ^–4  M Lyc可以显着提高公牛A和C的胚泡率及其IFN-tau表达。总之，补充1×10 ^–3  M VC或1×10 ^–4 冲洗和受精培养基中的M Lyc在IVF程序中极大地提高了按性别分选的公牛精子的受精能力。