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The need to freeze-Dehydration during specimen preparation for electron microscopy collapses the endothelial glycocalyx regardless of fixation method.
Microcirculation ( IF 2.4 ) Pub Date : 2020-06-16 , DOI: 10.1111/micc.12643
Casper Hempel 1, 2, 3 , Sergey Kapishnikov 4, 5 , Ana Joaquina Perez-Berna 6 , Stephan Werner 4 , Peter Guttmann 4 , Eva Pereiro 6 , Klaus Qvortrup 7 , Thomas Lars Andresen 1
Affiliation  

The endothelial glycocalyx covers the luminal surface of the endothelium and plays key roles in vascular function. Despite its biological importance, ideal visualization techniques are lacking. The current study aimed to improve the preservation and subsequent imaging quality of the endothelial glycocalyx.

中文翻译:

无论固定方法如何,在电子显微镜标本制备过程中都需要冷冻脱水,这会破坏内皮糖萼。

内皮糖萼覆盖内皮的腔表面,在血管功能中起着关键作用。尽管具有生物学重要性,但仍缺乏理想的可视化技术。目前的研究旨在改善内皮糖萼的保存和后续成像质量。
更新日期:2020-06-16
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