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Analysis of erythroid maturation in the nonlysed bone marrow with help of radar plots facilitates detection of flow cytometric aberrations in myelodysplastic syndromes.
Cytometry Part B: Clinical Cytometry ( IF 3.4 ) Pub Date : 2020-06-16 , DOI: 10.1002/cyto.b.21931
Despoina Violidaki 1, 2 , Olof Axler 1 , Katayoon Jafari 3 , Filippa Bild 1 , Lars Nilsson 4 , Joanna Mazur 5 , Mats Ehinger 1, 2 , Anna Porwit 1, 2
Affiliation  

Accumulating data support the role of flow cytometry (FCM) in diagnostic work‐up of myelodysplastic syndromes (MDS). Changes in erythropoiesis are less documented than in granulopoiesis. However, most studies were performed on bone marrow samples (BMSs) after red blood cell lysis. We have established a FCM protocol for erythropoiesis, following a no‐lysis approach and live gate acquisition of nucleated cells using DNA dye DRAQ5.

中文翻译:

借助雷达图分析未裂解骨髓中的红细胞成熟度,有助于检测骨髓增生异常综合征中的流式细胞仪畸变。

越来越多的数据支持流式细胞术 (FCM) 在骨髓增生异常综合征 (MDS) 的诊断检查中的作用。与粒细胞生成相比,红细胞生成的变化记录较少。然而,大多数研究是在红细胞裂解后对骨髓样本 (BMS) 进行的。我们已经建立了红细胞生成的 FCM 方案,遵循非裂解方法和使用 DNA 染料 DRAQ5 的有核细胞的活门采集。
更新日期:2020-06-16
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