Rapid and label-free electrochemical DNA biosensor based on a facile one-step electrochemical synthesis of rGO–PPy–(L-Cys)–AuNPs nanocomposite for the HTLV-1 oligonucleotide detection,Biotechnology and Applied Biochemistry

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Rapid and label-free electrochemical DNA biosensor based on a facile one-step electrochemical synthesis of rGO–PPy–(L-Cys)–AuNPs nanocomposite for the HTLV-1 oligonucleotide detection
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-06-15 , DOI: 10.1002/bab.1973
Mona Fani ₁ , Majid Rezayi _{2,

3} , Hamid R Pourianfar ₄ , Zahra Meshkat ₅ , Manoocher Makvandi ₁ , Mehrdad Gholami ₆ , Seyed Abdolrahim Rezaee ₇

Affiliation

Human T cell leukemia virus type 1 (HTLV-1) as the first human retrovirus is currently a serious endemic health challenge. Despite the use of assorted molecular or serological assays for HTLV-1 detection, there are several limitations due to the lack of a confirmatory test that may affect the accuracy of the results. Herein, a novel label-free biosensor for the detection of HTLV-1 Tax gene has been reported. An electrochemical facile ecofriendly synthesis method has been demonstrated based on a synthesis of nanocomposite of reduced graphene oxide, polypyrrole, and gold nanoparticles (rGO–PPy–(l-Cys)–AuNPs) deposited on the surface of screen-printed carbon electrode. Electrochemical techniques were used to characterize and study the electrochemical behavior of the rGO–PPy–(l-Cys)–AuNPs, which exhibited a stable reference peak at 0.21 V associated with hybridization forms by applying the differential pulse voltammetry. The designed DNA biosensor presented a wide linear range from 0.1 fM to 100 µM and a low detection limit of 20 atto-molar. The proposed biosensor presented in this study provides outstanding selectivity, sensitivity, repeatability, and reproducibility.

中文翻译：

基于 rGO-PPy-(L-Cys)-AuNPs 纳米复合材料的简单一步电化学合成的快速无标记电化学 DNA 生物传感器，用于 HTLV-1 寡核苷酸检测

人类 T 细胞白血病病毒 1 型 (HTLV-1) 作为第一种人类逆转录病毒，目前是严重的地方性健康挑战。尽管使用各种分子或血清学检测进行 HTLV-1 检测，但由于缺乏可能影响结果准确性的验证性测试，仍存在一些局限性。本文报道了一种用于检测 HTLV-1 Tax 基因的新型无标记生物传感器。基于沉积在丝网印刷碳电极表面上的还原氧化石墨烯、聚吡咯和金纳米颗粒（rGO-PPy-( l -Cys )-AuNPs）的纳米复合材料的合成，已经证明了一种电化学简便的环保合成方法。电化学技术用于表征和研究 rGO-PPy-( l-Cys)–AuNPs，通过应用差分脉冲伏安法，在 0.21 V 处表现出与杂交形式相关的稳定参考峰。设计的 DNA 生物传感器呈现从 0.1 fM 到 100 µM 的宽线性范围和 20 atto-molar 的低检测限。本研究中提出的生物传感器具有出色的选择性、灵敏度、重复性和再现性。

更新日期：2020-06-15

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