A novel fluorescent probe 4-(4′-(2-cyano-3-ethoxy-3-oxoprop-1-en-1-yl)- [1,1′-biphenyl]-4-yl)-2,2-difluoro-7,7-dimethyl-5,6,7,8-tetrahydro-2H-6,8-methanobenzo[d][1,3,2]dioxaborinin-1-ium-2-uide (CEFN) had been designed and developed for discriminative detection of N₂H₄ and HSO₃⁻ with two different sets of colorimetric and ratiometric fluorescence signals. The novel dual-response fluorescent probe CEFN can discriminative detect N₂H₄ and HSO₃⁻ with high selectivity, good sensitivity and fast response time. The response behaviors of probe CEFN for N₂H₄ and HSO₃⁻ were ascribed to different sensing mechanisms, which were investigated in detail by ¹H NMR, HRMS spectra. Furthermore, the probe CEFN could be served as a novel method for tracking N₂H₄ and HSO₃⁻ in living cells.

新型荧光探针4-（4'-（2-氰基-3-乙氧基-3-氧代丙-1-烯-1-基）-[1,1'-联苯] -4-基）-2,2-设计了二氟-7,7-二甲基-5,6,7,8-四氢-2H-6,8-甲氧基苯并[d] [1,3,2]二氧杂黄嘌呤-1-鎓-2-uide（CEFN）和对于N的判别检测开发₂ ħ ₄和HSO ₃ ^-用两组不同的比色和荧光比率信号。新颖的双响应荧光探针CEFN可以判别检测Ñ ₂ ħ ₄和HSO ₃ ^-以高选择性，良好的灵敏度和快速响应时间。CEFN探针对N ₂ H的响应行为₄和HSO ₃ ^-被归因于不同的感测机构，它是在详细研究了¹ H NMR，HRMS光谱。此外，探针CEFN可以充当用于跟踪N A新颖方法₂ ħ ₄和HSO ₃ ^-在活细胞。