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Biofabrication of zinc oxide nanoparticles from Melia azedarach and its potential in controlling soybean seed-borne phytopathogenic fungi.
Saudi Journal of Biological Sciences Pub Date : 2020-06-16 , DOI: 10.1016/j.sjbs.2020.06.013
T R Lakshmeesha 1, 2 , M Murali 3 , Mohammad Azam Ansari 4 , Arakere C Udayashankar 1 , Mohammad A Alzohairy 5 , Ahmad Almatroudi 5 , Mohammad N Alomary 6 , Sarah Mousa Maadi Asiri 7 , B S Ashwini 8 , Naveen Kumar Kalagatur 9 , Chandra S Nayak 1 , S R Niranjana 1
Affiliation  

Present study, report the biofabrication of zinc oxide nanoparticles from aqueous leaf extract of Melia azedarach (MaZnO-NPs) through solution combustion method and their novel application in preventing the growth of seed-borne fungal pathogens of soybean (Cladosporium cladosporioides and Fusarium oxysporum). The standard blotter method was employed to isolate fungi and was identified through molecular techniques. The characterization of MaZnO-NPs was carried out by UV–Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FT-IR), X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM) equipped with Energy Dispersive Spectroscopy (EDS) and Transmission Electron Microscopy (TEM). The physicochemical characterization confirmed the particles were of high purity and nano size (30–40 nm) with a hexagonal shape. The synthesized MaZnO-NPs inhibited the growth of C. cladosporioides and F. oxysporum in a dose dependent manner. Biomass, ergosterol, lipid peroxidation, intracellular reactive oxygen species and membrane integrity determination upon MaZnO-NPs treatment offered significant activities there by confirming the mechanism of action against the test pathogens. In conclusion, due to the effectiveness of MaZnO-NPs in controlling the growth of C. cladosporioides and F. oxysporum, the synthesized MaZnO-NPs provides insight towards their potential application in agriculture and food industries.



中文翻译:

Melia azedarach氧化锌纳米粒子的生物制造及其在控制大豆种子传播的植物致病真菌中的潜力。

本研究报道了通过溶液燃烧法从of水叶提取物(MaZnO-NPs)中生物制备氧化锌纳米颗粒及其在预防大豆种子传播真菌病原体(Cladosporium cladosporioidesFusarium oxysporum)生长中的新应用)。采用标准的印迹法分离真菌,并通过分子技术鉴定。通过紫外-可见光谱,傅立叶变换红外光谱(FT-IR),X射线衍射(XRD),配有能量色散谱(EDS)和透射电子的扫描电子显微镜(SEM)对MaZnO-NP进行表征显微镜(TEM)。物化特性证实颗粒具有高纯度和六边形的纳米尺寸(30-40 nm)。合成MaZnO纳米粒抑制的生长C.霉尖孢镰孢以剂量依赖的方式。MaZnO-NPs处理后的生物量,麦角固醇,脂质过氧化作用,细胞内活性氧种类和膜完整性测定通过确定针对测试病原体的作用机理,提供了重要的活性。总之,由于MaZnO-NPs在控制C. cladosporioidesF. oxysporum的生长中的有效性合成的MaZnO-NPs为他们在农业和食品工业中的潜在应用提供了见识。

更新日期:2020-06-16
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