Brucellosis, which is caused by Brucella spp., is an important zoonotic infectious disease that can cause great hazard to public health and safety. However, the current vaccines have several drawbacks, including residual virulence for animals and humans. Bacterial ghost is the empty envelopes of bacteria, which emerge as a proper vaccine candidate. With the purpose of generating B. abortus ghosts and investigating the immunogenicity of bacterial ghosts as vaccine candidate, we used homologous recombination and bacterial ghost technologies to construct 2308ΔgntR ghost strain. Mice were injected with 2308ΔgntR ghost and the safety and immunogenicity of ghost were further evaluated. The mice inoculated with ghost showed no splenomegaly. The 2308ΔgntR ghost induced high protective immunity in BALB/c mice against challenge with S2308, and elicited an anti-Brucella-specific immunoglobulin G (IgG) response and induced the secretion of interferon gamma (IFN-γ) and interleukin-4 (IL-4). Additionally, 2308ΔgntR ghost demonstrated strong spleen CD4⁺ and CD8⁺ T cell responses. These results suggest that 2308ΔgntR ghost is a potential vaccine candidate and may represent a promising new approach for vaccination against Brucella infection.

由布鲁氏菌属引起的布鲁氏菌病是一种重要的人畜共患性传染病，会对公众健康和安全造成极大危害。然而，当前的疫苗具有几个缺点，包括对动物和人类的残留毒力。细菌幽灵是空的细菌包膜，可作为合适的疫苗候选物出现。为了产生流产双歧杆菌幽灵并研究细菌幽灵作为候选疫苗的免疫原性，我们使用同源重组和细菌幽灵技术构建了2308ΔgntR幽灵菌株。给小鼠注射2308ΔgntR进一步评估了鬼影以及鬼影的安全性和免疫原性。接种幽灵的小鼠未见脾肿大。2308ΔgntR幽灵诱导BALB / c小鼠抵抗S2308攻击的高保护性免疫，并引发抗布鲁氏菌特异性免疫球蛋白G（IgG）反应，并诱导干扰素γ（IFN-γ）和白介素4（IL -4）。此外，2308Δ gntR鬼表现出很强的脾CD4 ⁺和CD8 ⁺ T细胞应答。这些结果表明2308ΔgntR幽灵是潜在的疫苗候选者，并且可能代表了针对布鲁氏菌感染的疫苗接种的有前途的新方法。