Cinnamomum camphora has great economic value and produces abundant of monoterpenes to improve the thermotolerance. To uncover the thermotolerance mechanisms of main functional monoterpenes for developing anti-high temperature agents, the reactive oxygen species (ROS) metabolism, photosynthetic abilities and gene expression were investigated in the plant leaves fumigated with 2 main monoterpenes (terpinene and β-pinene) under high temperature (38 °C) with fosmidomycin (Fos) inhibiting monoterpene synthesis (Fos+38 °C + terpinene and Fos+38 °C+β-pinene). High temperature (38 °C) increased the ROS and thiobarbituric acid reactive substance (TBARS) content as well as antioxidant enzyme activities, but declined the photosynthetic abilities. These variations were further aggravated under 38 °C with Fos pretreatment (Fos+38 °C), but were gradually recovered with increasing the monoterpene concentration in Fos+38 °C + terpinene and Fos+38 °C+β-pinene treatments. The expression of 73 genes involving in non-enzymatic antioxidant metabolism, photosynthesis and other pathways was changed in Fos+38 °C treatment. Interestingly, the occurrence of 10 μM terpinene and/ or β-pinene showed recovery effects on the expression of these altered genes. Therefore, terpinene and β-pinene were 2 main functional monoterpenes to improve C. camphora thermotolerance by adjusting gene expression.

樟树具有巨大的经济价值，并产生大量的单萜以提高耐热性。为了揭示主要功能性单萜类化合物开发抗高温剂的耐热机制，研究了在2种主要单萜类化合物（萜品烯和β-pine烯）熏蒸下的植物叶片中活性氧（ROS）代谢，光合能力和基因表达。高温（38°C）和磷霉素（Fos）抑制单萜合成（Fos + 38°C +松油烯和Fos + 38°C +β-pine烯）。高温（38°C）增加了ROS和硫代巴比妥酸反应性物质（TBARS）的含量以及抗氧化酶的活性，但降低了光合能力。在Fos预处理（Fos + 38°C）下，这些变化在38°C下进一步加剧，但在Fos + 38°C +萜品烯和Fos + 38°C +β-pine烯处理中，随着单萜浓度的增加逐渐恢复。Fos + 38°C处理改变了涉及非酶抗氧化代谢，光合作用等途径的73个基因的表达。有趣的是，10μM萜品烯和/或β-pine烯的出现显示出对这些改变的基因表达的恢复作用。因此，萜品烯和β-pine烯是改善的两个主要功能单萜 10μM松油烯和/或β-pine烯的出现显示出对这些改变的基因表达的恢复作用。因此，萜品烯和β-pine烯是改善的两个主要功能单萜 10μM松油烯和/或β-pine烯的出现显示出对这些改变的基因表达的恢复作用。因此，萜品烯和β-pine烯是改善的两个主要功能单萜樟脑耐热性通过调节基因表达来实现。