Sapium sebiferum Roxb. is an economically important multipurpose woody plant in terms of its biodiesel-producing, medicinal and ornamental values. In the present study, a high throughput in vitro regeneration system of S. sebiferum through direct shoot organogenesis from shoot stems was developed. The effects of plant growth regulators (PGRs), explant orientation, culture vessel type, explant type and explant source on adventitious shoot induction were investigated. Adventitious shoots were induced from cut ends of shoot stems with or without axillary buds when they were cultivated on the Murashige and Skoog (MS) medium fortified with 6-benzylaminopurine (6-BA). The highest rate of shoot induction (100 %) with mean number of shoot buds (21.4 ± 0.2) per explant was obtained when in vitro shoot stems without axillary buds were placed horizontally on the medium supplemented with 0.5 mg l⁻¹ 6-BA in glass petri dish. The induced shoot buds were transferred to MS medium supplemented with 0.5 mg l⁻¹ 6-BA and 0.1 mg l⁻¹ gibberellic acid (GA₃) for shoot proliferation and elongation. The elongated shoots were continuously propagated through shoot stem sectioning until used for rooting. For in vitro adventitious root induction, the elongated shoots were cultivated on the MS basal medium supplemented with different strengths of macronutrient, various concentrations of auxins and sucrose. The highest root induction (100 %) was achieved when the elongated shoots were cultured on 1/8 MS medium fortified with 2.0 % sucrose and 0.5 mg l^-1 indole-3-butyric acid (IBA). For ex vitro rooting, 98.3 % shoots rooted when they were pretreated with IBA (1000 mg l^-1) for 60 s. The plantlets rooted by both methods were successfully acclimatized and showed normal development with 91.4 % and 98.1 % survival rate, respectively. The genetic fidelity of the regenerants was confirmed by inter simple sequence repeat (ISSR) markers. The present in vitro regeneration protocol will play an important role in large-scale propagation, germplasm conservation as well as genetic improvement of this economically important multipurpose plant species.

Sapium sebiferum Roxb。就生物柴油的生产，药用和观赏价值而言，它是一种经济上重要的多用途木本植物。在本研究中，高通量的S. sebiferum体外再生系统通过从芽茎直接芽发生器官发育。研究了植物生长调节剂（PGRs），外植体取向，培养容器类型，外植体类型和外植体来源对不定芽诱导的影响。不定芽是在具有6-苄基氨基嘌呤（6-BA）的Murashige和Skoog（MS）培养基上培养时，从有或没有腋芽的芽茎的切端诱导出来的。当将无腋芽的体外芽茎水平放置在补充有0.5 mg l ^-1 6-BA的培养基中时，可获得最高的芽诱导率（100％），平均每个外植芽的芽芽数（21.4±0.2）。玻璃培养皿。将诱导的芽芽转移到补充有0.5 mg l ^-1的MS培养基中6-BA和0.1 mg l ^-1赤霉素（GA ₃）用于芽的增殖和伸长。细长的枝条通过茎干切片连续繁殖，直至生根。对于体外不定根诱导，将伸长的芽在补充了不同强度的常量营养素，各种浓度的生长素和蔗糖的MS基础培养基上培养。当将伸长的枝条在以2.0％蔗糖和0.5 mg ^-1吲哚-3-丁酸（IBA）强化的1/8 MS培养基上培养时，可获得最高的根诱导（100％）。对于离体生根，当用IBA（1000 mg l ^-1）预处理时，有98.3％的芽生根）60秒。通过这两种方法生根的小植株均已成功驯化，并显示出正常发育，存活率分别为91.4％和98.1％。通过内部简单序列重复（ISSR）标记确认了再生子的遗传保真度。当前的体外再生方案将在这种经济上重要的多用途植物物种的大规模繁殖，种质保护以及遗传改良中发挥重要作用。