Extracellular vesicles (EVs) have been recognized as vital mediators of intercellular communication that allow horizontal information exchange among tumor cells as well as reciprocal cross-talk between tumor and stromal cells. Long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) are selectively packaged into EVs then transferred to proximal and distal recipient cells, inducing profound phenotypic changes. Recently, there has been substantial progress in understanding the essential roles of EV-associated lncRNAs and circRNAs in cancer initiation and progression. The distinctive properties of these ncRNAs carried by EVs, including their tissue-specific expression, relative stability, targeted delivery and widespread presence in various biofluids, has also prompted the exploration of their potential in liquid biopsy and therapeutic usage. Herein, we summarized the biology, characteristics and functions of EV-associated lncRNAs and circRNAs, highlighting their vital roles in hallmarks of cancer and their prospective applications in cancer management. The challenges unresolved in this field were additionally pointed out to guide future studies.

细胞外囊泡（EVs）已被认为是细胞间通讯的重要介质，它允许肿瘤细胞之间进行水平信息交换以及肿瘤与基质细胞之间的相互干扰。将长的非编码RNA（lncRNA）和环状RNA（circRNA）选择性包装到EV中，然后转移到近端和远端受体细胞中，引起深刻的表型变化。最近，在了解与EV相关的lncRNA和circRNA在癌症的发生和发展中的重要作用方面已经取得了实质性进展。EV携带的这些ncRNA的独特特性，包括它们的组织特异性表达，相对稳定性，靶向递送和在各种生物流体中的广泛存在，也促使人们探索其在液体活检和治疗用途中的潜力。在这里，我们总结了与EV相关的lncRNA和circRNA的生物学，特性和功能，突出了它们在癌症标志中的重要作用及其在癌症管理中的前瞻性应用。另外指出了该领域尚未解决的挑战，以指导未来的研究。