In natural environments, organisms are often exposed to several environmental pollutants at any one time, and the potential effects of such co-exposures on human and environmental health are of considerable concern. It is thought that multi-walled carbon nanotubes (MWCNTs) may interact with other pollutants in aquatic systems and induce considerably different effects compared with exposure to a single contaminant. The objective of this study was to evaluate the potential acute combined effects of mixtures of MWCNTs and 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) on embryonic development stages, oxidative stress, apoptosis and DNA damage in developing zebrafish (Danio rerio). The embryos were treated with BDE-47 (5, 10, and 50 μg/L) and MWCNTs (50 mg/L), either combined or individually, for 96 h. Following exposure, BDE-47 induced significant acute toxicity, while the MWCNTs exhibited slight toxicity. When compared with BDE-47-only exposure, the inhibited growth induced by BDE-47 was weakened in the presence of MWCNTs. Similarly, the levels of oxidative stress biomarkers (reactive oxygen species, superoxide dismutase, catalase activities and malondialdehyde), apoptosis (apoptosis rate, caspase-3 and caspase-9 activities) and DNA damage (comet assay and comet olive tails) decreased in the presence of MWCNTs compared to those exposed to BDE-47 alone. These results demonstrate that MWCNTs can weaken the developmental inhibition, oxidative stress, apoptosis and DNA damage induced by BDE-47 in the early stages of zebrafish development.

在自然环境中，生物通常随时都暴露于多种环境污染物中，因此，此类共同暴露对人类和环境健康的潜在影响是相当令人关注的。据认为，与暴露于单一污染物相比，多壁碳纳米管（MWCNT）可能会与水生系统中的其他污染物相互作用，并产生明显不同的影响。这项研究的目的是评估MWCNTs和2,2'，4,4'-四溴二苯醚（BDE-47）混合物对发育中的斑马鱼胚胎发育阶段，氧化应激，细胞凋亡和DNA损伤的潜在急性联合作用。 （Danio rerio）。胚胎分别用BDE-47（5、10和50μg/ L）和MWCNT（50 mg / L）组合或单独处理96小时。暴露后，BDE-47引起明显的急性毒性，而MWCNT表现出轻微的毒性。与仅含BDE-47的暴露相比，在MWCNT的存在下，由BDE-47诱导的抑制生长减弱。同样，氧化应激生物标志物（活性氧，超氧化物歧化酶，过氧化氢酶活性和丙二醛），细胞凋亡（凋亡率，caspase-3和caspase-9活性）和DNA损伤（彗星试验和彗星橄榄色尾巴）的水平降低。与仅暴露于BDE-47的那些相比存在MWCNT。这些结果表明，MWCNTs可以减弱发育抑制，氧化应激，