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Antagonistic effects of multi-walled carbon nanotubes and BDE-47 in zebrafish (Danio rerio): Oxidative stress, apoptosis and DNA damage.
Aquatic Toxicology ( IF 4.1 ) Pub Date : 2020-06-16 , DOI: 10.1016/j.aquatox.2020.105546
Weitong Wang 1 , Xuesong Zhao 1 , Xin Ren 2 , Xiaoyue Duan 1
Affiliation  

In natural environments, organisms are often exposed to several environmental pollutants at any one time, and the potential effects of such co-exposures on human and environmental health are of considerable concern. It is thought that multi-walled carbon nanotubes (MWCNTs) may interact with other pollutants in aquatic systems and induce considerably different effects compared with exposure to a single contaminant. The objective of this study was to evaluate the potential acute combined effects of mixtures of MWCNTs and 2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) on embryonic development stages, oxidative stress, apoptosis and DNA damage in developing zebrafish (Danio rerio). The embryos were treated with BDE-47 (5, 10, and 50 μg/L) and MWCNTs (50 mg/L), either combined or individually, for 96 h. Following exposure, BDE-47 induced significant acute toxicity, while the MWCNTs exhibited slight toxicity. When compared with BDE-47-only exposure, the inhibited growth induced by BDE-47 was weakened in the presence of MWCNTs. Similarly, the levels of oxidative stress biomarkers (reactive oxygen species, superoxide dismutase, catalase activities and malondialdehyde), apoptosis (apoptosis rate, caspase-3 and caspase-9 activities) and DNA damage (comet assay and comet olive tails) decreased in the presence of MWCNTs compared to those exposed to BDE-47 alone. These results demonstrate that MWCNTs can weaken the developmental inhibition, oxidative stress, apoptosis and DNA damage induced by BDE-47 in the early stages of zebrafish development.



中文翻译:

斑马鱼中多壁碳纳米管和BDE-47的拮抗作用:氧化应激,细胞凋亡和DNA损伤。

在自然环境中,生物通常随时都暴露于多种环境污染物中,因此,此类共同暴露对人类和环境健康的潜在影响是相当令人关注的。据认为,与暴露于单一污染物相比,多壁碳纳米管(MWCNT)可能会与水生系统中的其他污染物相互作用,并产生明显不同的影响。这项研究的目的是评估MWCNTs和2,2',4,4'-四溴二苯醚(BDE-47)混合物对发育中的斑马鱼胚胎发育阶段,氧化应激,细胞凋亡和DNA损伤的潜在急性联合作用。 (Danio rerio)。胚胎分别用BDE-47(5、10和50μg/ L)和MWCNT(50 mg / L)组合或单独处理96小时。暴露后,BDE-47引起明显的急性毒性,而MWCNT表现出轻微的毒性。与仅含BDE-47的暴露相比,在MWCNT的存在下,由BDE-47诱导的抑制生长减弱。同样,氧化应激生物标志物(活性氧,超氧化物歧化酶,过氧化氢酶活性和丙二醛),细胞凋亡(凋亡率,caspase-3和caspase-9活性)和DNA损伤(彗星试验和彗星橄榄色尾巴)的水平降低。与仅暴露于BDE-47的那些相比存在MWCNT。这些结果表明,MWCNTs可以减弱发育抑制,氧化应激,

更新日期:2020-06-23
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