This work investigates the feasibility of growing marine microalgae Nannochloropsis oculata in a mix of seawater and saline produced water obtained during oil & gas extraction, supplemented with liquid digestate from anaerobic digestion process as source of nutrients. In particular, three-stage cultures were conducted by varying the produced water loading in the culture media (from 0 up to 50% v/v), supplemented with 5% v/v of digestate and seawater. Growth parameters as well as nitrogen (ammonium) and organic carbon (expressed as chemical oxygen demand) removal efficiencies were monitored. Results revealed that N. oculata is perfectly able to grow in a seawater containing produced water from 10 up to 30% v/v. A slower growth was observed for 40 and 50% v/v of produced water, because of the salinity higher than 60 g·L⁻¹. Maximal growth rates obtained were 0.35, 0.27 and 0.16 day⁻¹, with a maximal optical density of 6.3, 5.2 and 3.2 for 10, 20 and 30% v/v of produced water, respectively. Nannochloropsis oculata showed better removal efficiencies for ammonium nitrogen (around 100%) than for organic carbon (approximately 40% after one step of acclimation), regardless of the produced water loading, most chemical oxygen demand being volatilized and/or degraded by bacteria during the first two days of a culture. Regardless of the loading, >90% of iron brought by produced water and digestate was precipitated and/or assimilated/adsorbed by N. oculata.

这项工作研究了在油气提取过程中获得的海水和盐水生产水的混合物中生长海洋微藻Nannochloropsis oculata的可行性，并补充了厌氧消化过程中产生的液体消化物作为养分的来源。特别地，通过改变培养基中产生的水负荷（从0到50％v / v），并补充5％v / v的消化液和海水，进行三阶段培养。监测生长参数以及氮（铵）和有机碳（表示为化学需氧量）的去除效率。结果显示，猪笼草完全能够在含10％至30％v / v采出水的海水中生长。由于矿化度高于60 g·L ^-1，观察到40％和50％v / v的产出水生长较慢。对于10％，20％和30％v / v的生产水，获得的最大生长速率分别为0.35、0.27和0.16天^-1，最大光密度分别为6.3、5.2和3.2。拟南芥表现出对铵态氮（约100％）的去除效率要比对有机碳（一个适应步骤后约40％）的去除效率更高，无论产生的水量如何，前两个阶段中大多数化学需氧量被细菌挥发和/或降解文化的日子。不管装载的，> 90％的所产生的水和消化带来铁的沉淀和/或同化/通过吸附微拟球藻。