Abstract

Microalgae are important biological resources which attract the researchers for the production of valuable products like bio-energy and value-added products. In this study, enhanced accumulation of carotenoids and lipids was obtained from Coelastrella sp. M60 which was grown at 40 °C, along with different stress conditions (nitrogen starvation, salinity and TiO₂). The results clearly indicate that osmotic stress and nitrogen starvation along with high temperature had induced the yield of zeaxanthin (13.15 mg/g DCW) and the lipid content (39.51 ± 5.13%). Further, it was also observed that TiO₂ combined with other stresses had induced the increased accumulation of astaxanthin and zeaxanthin up to 1.16 and 0.51 fold on the 10th day of cultivation. On the contrary, TiO₂, along with other stress conditions had influenced the biomass production up to 1.16 fold than the control, but no detectable increase in lipid content was observed. Thus, for the first time it is reported that by employing combined stress factors such as high temperature, nitrogen starvation and 3% salinity had resulted in significant improvement in zeaxanthin production in the foresaid microalgae. Further, it was also noticed that the Coelastrella sp. M60 had accumulated the carotenoids and lipids under TiO₂ along with other oxidative stress conditions within the short duration.

Graphic abstract

中文翻译：

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作为增强剂的光催化剂（TiO 2）：尝试通过在Coelastrella sp。中组合氧化应激来增强类胡萝卜素和脂质的产生。M60

摘要

微藻是重要的生物资源，吸引了研究人员用于生产有价值的产品，如生物能源和增值产品。在这项研究中，类固醇和脂质的积累从Coelastrella sp。获得。M60在40°C下生长，并带有不同的应力条件（氮饥饿，盐度和TiO ₂）。结果清楚地表明，渗透胁迫和氮饥饿以及高温诱导了玉米黄质的产量（13.15 mg / g DCW）和脂质含量（39.51±5.13％）。此外，还观察到TiO ₂在培养的第10天，加上其他胁迫，虾青素和玉米黄质的累积积累增加了1.16和0.51倍。相反，TiO ₂以及其他胁迫条件对生物量产生的影响比对照大1.16倍，但未观察到脂质含量的可检测的增加。因此，首次报道了通过使用诸如高温，氮饥饿和3％盐度的综合胁迫因素，已使上述微藻类中的玉米黄质产生显着改善。此外，还发现了腔棘鱼。M60在短时间内在TiO ₂和其他氧化应激条件下积累了类胡萝卜素和脂质。

图形摘要