The cost of culture media represents a considerable portion of the overall cost in syngas fermentation. Yeast extract is an expensive ingredient among the medium components required to prepare the culture medium. In this study, we evaluated the effectiveness of replacing yeast extract with relatively low-cost nutrient supplements such as corn steep liquor, malt extract, and vegetable extract in improving the feasibility of the syngas fermentation process in Clostridium autoethanogenum culture. The use of corn steep liquor, malt extract, and vegetable extract as nutrient supplements at a concentration of 0.5 g/L resulted in optical densities of 1.44, 1.37, and 1.71, and produced ethanol concentrations of 2.24, 3.37, and 3.76 g/L, respectively. The microbial growth and ethanol production in syngas fermentation with yeast extract alternatives was comparable with the results of fermentation with yeast extract (optical density of 1.48 and ethanol concentration of 2.16). Moreover, the use of malt and vegetable extracts showed considerable improvement (69% and 51%, respectively) in the specific ethanol productivity compared to yeast extract.

培养基的成本占合成气发酵总成本的相当一部分。酵母提取物是制备培养基所需的培养基成分中的昂贵成分。在这项研究中，我们评估了用相对低成本的营养补充剂（例如玉米浆，麦芽提取物和蔬菜提取物）代替酵母提取物在提高自产乙醇梭菌中合成气发酵过程的可行性方面的有效性。文化。以0.5 g / L的浓度使用玉米浆，麦芽提取物和蔬菜提取物作为营养补充剂时，光密度为1.44、1.37和1.71，乙醇浓度为2.24、3.37和3.76 g / L ， 分别。酵母提取物替代品在合成气发酵中的微生物生长和乙醇产生与酵母提取物发酵的结果相当（光密度为1.48，乙醇浓度为2.16）。此外，与酵母提取物相比，麦芽和蔬菜提取物的使用显示出比乙醇生产率的显着提高（分别为69％和51％）。