Abstract

Bacterial magnetic particles (BMPs) are biosynthesized magnetic nano-scale materials with excellent dispersibility and biomembrane enclosure properties. In this study, we demonstrate that BMPs augment the ability of polyethylenimine (PEI) to deliver target DNA into difficult-to-transfect primary porcine liver cells, with transfection efficiency reaching over 30%. Compared with standard lipofection and polyfection, BMP-PEI gene vectors significantly enhanced the transfection efficiencies for the primary porcine liver cells and C2C12 mouse myoblast cell lines. To better understand the mechanism of magnetofection using BMP-PEI/DNA vectors, transmission electron microscopy (TEM) images of transfected Cos-7, HeLa, and HEP-G2 cells were observed. We found that the BMP-PEI/DNA complexes were trafficked into the cytoplasm and nucleus by way of vesicular transport and endocytosis. Our study builds support for the versatile BMP-PEI vector transfection system, which might be exploited to transfect a wide range of cell types or even to reach specific targets in the treatment of disease.

Key Points

• We constructed a BMP-PEI gene delivery vector by combining BMPs and PEI.

• The vector significantly enhanced transfection efficiencies in eukaryotic cell lines.

• The transfection mechanism of this vector was explained in our study.

中文翻译：

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细菌磁性颗粒-聚乙烯亚胺载体可高效，低毒地将靶基因传递到多种细胞类型中。

摘要

细菌磁性颗粒（BMP）是生物合成的磁性纳米级材料，具有出色的分散性和生物膜包裹性。在这项研究中，我们证明BMP增强了聚乙烯亚胺（PEI）的能力，可将目标DNA输送到难以转染的原代猪肝细胞中，转染效率可达到30％以上。与标准脂质转染和多重转染相比，BMP-PEI基因载体显着提高了原代猪肝细胞和C2C12小鼠成肌细胞系的转染效率。为了更好地了解使用BMP-PEI / DNA载体进行磁转染的机制，观察了转染的Cos-7，HeLa和HEP-G2细胞的透射电子显微镜（TEM）图像。我们发现BMP-PEI / DNA复合物通过水泡运输和内吞作用被贩运到细胞质和细胞核中。我们的研究为多功能的BMP-PEI载体转染系统提供了支持，该系统可被用于转染多种细胞类型，甚至达到治疗疾病的特定目标。

关键点

•我们通过结合BMP和PEI构建了BMP-PEI基因递送载体。

•该载体显着提高了真核细胞系的转染效率。

•在我们的研究中解释了该载体的转染机制。