Changes of soil microbial communities are one of the main factors of continuous cropping problem. Andrographis paniculata has been reported to have replant problem in cultivation. However, little is known about the variations of rhizosphere soil microbial communities of A. paniculata under a continuous cropping system. Here, Illumina MiSeq was used to investigate the shifts of rhizospheric bacterial and fungal communities after continuous cropping of A. paniculata. The bacterial diversity increased whereas the fungal diversity decreased in rhizosphere soil after consecutive A. paniculata monoculture; and the soil microbial community structure differed between newly plant soil and continuous cropped soil. Taxonomic analyses further revealed that the bacterial phyla Proteobacteria, Acidobacteria and Bacteroidetes and the fungal phyla Zygomycota, Ascomycota and Cercozoa were the dominant phyla across all soil samples. The relative abundance of phyla Acidobacteria and Zygomycota were significantly increased after continuous cropping. Additionally, the most abundant bacterial genus Pseudolabrys significantly decreased, while the predominant fungal genus Mortierella increased considerably in abundance after continuous cropping. Our results revealed the changes on diversity and composition of bacterial and fungal communities in rhizospheric soil under continuous cropping of A. paniculata. These data contributed to the understanding of soil micro-ecological environments in the rhizosphere of A. paniculata.

中文翻译：

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穿心莲连续种植方式对根际土壤微生物群落的影响。

土壤微生物群落的变化是连作问题的主要因素之一。据报道穿心莲有种植中的补种问题。然而，关于连作模式下沙地土壤根际土壤微生物群落的变化知之甚少。在这里，Illumina MiSeq用于调查连续种植沙棘曲霉后根际细菌和真菌群落的变化。连续进行沙棘曲霉单培养后，根际土壤细菌多样性增加，而真菌多样性减少。新种植土壤与连作土壤之间的微生物群落结构不同。分类学分析进一步表明，细菌门菌是Proteobacteria，Acidobacteria和Bacteroidetes以及真菌门系Zygomycota，在所有土壤样品中，子囊菌和尾er均是优势菌。连作后，酸性细菌和合子菌的相对丰度明显增加。此外，连续种植后，最丰富的细菌属假单胞菌显着减少，而主要的真菌属Mortierella则大量增加。我们的研究结果表明，连续种植紫穗槐时，根际土壤细菌和真菌群落的多样性和组成发生了变化。这些数据有助于了解A. paniculata的根际土壤微生态环境。此外，连续种植后，最丰富的细菌属假单胞菌显着减少，而主要的真菌属Mortierella则大量增加。我们的研究结果表明，连续种植紫穗槐时，根际土壤细菌和真菌群落的多样性和组成发生了变化。这些数据有助于了解A. paniculata的根际土壤微生态环境。此外，连续种植后，最丰富的细菌属假单胞菌显着减少，而主要的真菌属Mortierella则大量增加。我们的研究结果表明，连续种植紫穗槐时，根际土壤细菌和真菌群落的多样性和组成发生了变化。这些数据有助于了解A. paniculata的根际土壤微生态环境。