A facile enzymatic modular assembly strategy for the preparative-scale synthesis of poly-N-acetyllactosamine (poly-LacNAc) glycans with varied lengths and designed sialylation and/or fucosylation patterns is described. These glycans were printed as a microarray to investigate their interactions with a panel of glycan binding proteins (GBPs). Binding affinities revealed that the avidity of GBPs could be largely affected by the length and the patterns of sialylation and fucosylation.

中文翻译：

---

聚N-乙酰基乳糖胺衍生物的酶促模块合成和微阵列分析。

描述了用于制备规模合成长度可变和设计的唾液酸化和/或岩藻糖基化模式的聚-N-乙酰基乳糖胺（poly-LacNAc）聚糖的简便酶促模块组装策略。这些聚糖被印刷为微阵列，以研究它们与一组聚糖结合蛋白（GBP）的相互作用。结合亲和力表明，GBPs的亲和力在很大程度上受唾液酸化和岩藻糖基化的长度和模式影响。